Quantitation of cholesterol crystallization from supersaturated model bile

B. Piero Portincasaa, Niels G. Vennemana, B. Antonio Moschettaa, André Den Van Bergc, Giuseppe Palascianob, Gerard P. Vanberge-Henegouwena, Karel J. Van Erpecuma

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Cholesterol crystallization is an essential step in gallstone formation. Although spectrophotometry and nephelometry have been used for quantitation of crystallization, potential effects of crystal size and shape have not been evaluated. We determined crystallization in model biles [total lipid concentration 7.3 g/dl, egg yolk Phosphatidylcholine (EYPC)/(EYPC+taurocholate) molar ratio = 0.05, 0.15, or 0.30; cholesterol saturation index (CSI) = 1.2, 1.7, or 2.0; 37°C] plotting in the central three-phase (micelles, vesicles, and crystals containing) zone or in the left two-phase (micelles and crystals containing) zone of the equilibrium ternary phase diagram. Extent of crystallization estimated by spectrophotometry and nephelometry was related to chemical determination of crystal mass and to crystal size or shape (by microscopy). With all methods, crystallization was less extensive when vesicles were present (central three-phase zone) and at lower CSIs. In the left two-phase zone, particularly at EYPC/(EYPC+taurocholate), ratio of 0.15, there were strong increases in spectrophotometric and nephelometric readings during the first days of incubation, but decreases at later stages, despite progressive increases in crystal mass by chemical measurement. Initially, there were large numbers of very small crystals (<10 μm) in these biles, which were subsequently replaced by large cholesterol monohydrate crystals. Decreasing sizes of harvested cholesterol monohydrate crystals by sonication increased spectrophotometric and nephelometric values despite identical crystal mass. When cholesterol crystal mass is assayed by indirect methods such as spectrophotometry or nephelometry, results are strongly influenced by crystal size.

Original languageEnglish (US)
Pages (from-to)604-610
Number of pages7
JournalJournal of Lipid Research
Volume43
Issue number4
StatePublished - 2002

Fingerprint

Crystallization
Egg Yolk
Bile
Nephelometry and Turbidimetry
Phosphatidylcholines
Cholesterol
Spectrophotometry
Crystals
Taurocholic Acid
Micelles
Sonication
Gallstones
Reading
Microscopy
Lipids
Phase diagrams
Microscopic examination

Keywords

  • Bile salts
  • Gallstone
  • Micelles
  • Microscopy
  • Nephelometry
  • Phosphatidylcholine
  • Phospholipids
  • Spectrophotometry
  • Taurocholate
  • Vesicles

ASJC Scopus subject areas

  • Endocrinology

Cite this

Piero Portincasaa, B., Vennemana, N. G., Antonio Moschettaa, B., Van Bergc, A. D., Palascianob, G., Vanberge-Henegouwena, G. P., & Van Erpecuma, K. J. (2002). Quantitation of cholesterol crystallization from supersaturated model bile. Journal of Lipid Research, 43(4), 604-610.

Quantitation of cholesterol crystallization from supersaturated model bile. / Piero Portincasaa, B.; Vennemana, Niels G.; Antonio Moschettaa, B.; Van Bergc, André Den; Palascianob, Giuseppe; Vanberge-Henegouwena, Gerard P.; Van Erpecuma, Karel J.

In: Journal of Lipid Research, Vol. 43, No. 4, 2002, p. 604-610.

Research output: Contribution to journalArticle

Piero Portincasaa, B, Vennemana, NG, Antonio Moschettaa, B, Van Bergc, AD, Palascianob, G, Vanberge-Henegouwena, GP & Van Erpecuma, KJ 2002, 'Quantitation of cholesterol crystallization from supersaturated model bile', Journal of Lipid Research, vol. 43, no. 4, pp. 604-610.
Piero Portincasaa B, Vennemana NG, Antonio Moschettaa B, Van Bergc AD, Palascianob G, Vanberge-Henegouwena GP et al. Quantitation of cholesterol crystallization from supersaturated model bile. Journal of Lipid Research. 2002;43(4):604-610.
Piero Portincasaa, B. ; Vennemana, Niels G. ; Antonio Moschettaa, B. ; Van Bergc, André Den ; Palascianob, Giuseppe ; Vanberge-Henegouwena, Gerard P. ; Van Erpecuma, Karel J. / Quantitation of cholesterol crystallization from supersaturated model bile. In: Journal of Lipid Research. 2002 ; Vol. 43, No. 4. pp. 604-610.
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abstract = "Cholesterol crystallization is an essential step in gallstone formation. Although spectrophotometry and nephelometry have been used for quantitation of crystallization, potential effects of crystal size and shape have not been evaluated. We determined crystallization in model biles [total lipid concentration 7.3 g/dl, egg yolk Phosphatidylcholine (EYPC)/(EYPC+taurocholate) molar ratio = 0.05, 0.15, or 0.30; cholesterol saturation index (CSI) = 1.2, 1.7, or 2.0; 37°C] plotting in the central three-phase (micelles, vesicles, and crystals containing) zone or in the left two-phase (micelles and crystals containing) zone of the equilibrium ternary phase diagram. Extent of crystallization estimated by spectrophotometry and nephelometry was related to chemical determination of crystal mass and to crystal size or shape (by microscopy). With all methods, crystallization was less extensive when vesicles were present (central three-phase zone) and at lower CSIs. In the left two-phase zone, particularly at EYPC/(EYPC+taurocholate), ratio of 0.15, there were strong increases in spectrophotometric and nephelometric readings during the first days of incubation, but decreases at later stages, despite progressive increases in crystal mass by chemical measurement. Initially, there were large numbers of very small crystals (<10 μm) in these biles, which were subsequently replaced by large cholesterol monohydrate crystals. Decreasing sizes of harvested cholesterol monohydrate crystals by sonication increased spectrophotometric and nephelometric values despite identical crystal mass. When cholesterol crystal mass is assayed by indirect methods such as spectrophotometry or nephelometry, results are strongly influenced by crystal size.",
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T1 - Quantitation of cholesterol crystallization from supersaturated model bile

AU - Piero Portincasaa, B.

AU - Vennemana, Niels G.

AU - Antonio Moschettaa, B.

AU - Van Bergc, André Den

AU - Palascianob, Giuseppe

AU - Vanberge-Henegouwena, Gerard P.

AU - Van Erpecuma, Karel J.

PY - 2002

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N2 - Cholesterol crystallization is an essential step in gallstone formation. Although spectrophotometry and nephelometry have been used for quantitation of crystallization, potential effects of crystal size and shape have not been evaluated. We determined crystallization in model biles [total lipid concentration 7.3 g/dl, egg yolk Phosphatidylcholine (EYPC)/(EYPC+taurocholate) molar ratio = 0.05, 0.15, or 0.30; cholesterol saturation index (CSI) = 1.2, 1.7, or 2.0; 37°C] plotting in the central three-phase (micelles, vesicles, and crystals containing) zone or in the left two-phase (micelles and crystals containing) zone of the equilibrium ternary phase diagram. Extent of crystallization estimated by spectrophotometry and nephelometry was related to chemical determination of crystal mass and to crystal size or shape (by microscopy). With all methods, crystallization was less extensive when vesicles were present (central three-phase zone) and at lower CSIs. In the left two-phase zone, particularly at EYPC/(EYPC+taurocholate), ratio of 0.15, there were strong increases in spectrophotometric and nephelometric readings during the first days of incubation, but decreases at later stages, despite progressive increases in crystal mass by chemical measurement. Initially, there were large numbers of very small crystals (<10 μm) in these biles, which were subsequently replaced by large cholesterol monohydrate crystals. Decreasing sizes of harvested cholesterol monohydrate crystals by sonication increased spectrophotometric and nephelometric values despite identical crystal mass. When cholesterol crystal mass is assayed by indirect methods such as spectrophotometry or nephelometry, results are strongly influenced by crystal size.

AB - Cholesterol crystallization is an essential step in gallstone formation. Although spectrophotometry and nephelometry have been used for quantitation of crystallization, potential effects of crystal size and shape have not been evaluated. We determined crystallization in model biles [total lipid concentration 7.3 g/dl, egg yolk Phosphatidylcholine (EYPC)/(EYPC+taurocholate) molar ratio = 0.05, 0.15, or 0.30; cholesterol saturation index (CSI) = 1.2, 1.7, or 2.0; 37°C] plotting in the central three-phase (micelles, vesicles, and crystals containing) zone or in the left two-phase (micelles and crystals containing) zone of the equilibrium ternary phase diagram. Extent of crystallization estimated by spectrophotometry and nephelometry was related to chemical determination of crystal mass and to crystal size or shape (by microscopy). With all methods, crystallization was less extensive when vesicles were present (central three-phase zone) and at lower CSIs. In the left two-phase zone, particularly at EYPC/(EYPC+taurocholate), ratio of 0.15, there were strong increases in spectrophotometric and nephelometric readings during the first days of incubation, but decreases at later stages, despite progressive increases in crystal mass by chemical measurement. Initially, there were large numbers of very small crystals (<10 μm) in these biles, which were subsequently replaced by large cholesterol monohydrate crystals. Decreasing sizes of harvested cholesterol monohydrate crystals by sonication increased spectrophotometric and nephelometric values despite identical crystal mass. When cholesterol crystal mass is assayed by indirect methods such as spectrophotometry or nephelometry, results are strongly influenced by crystal size.

KW - Bile salts

KW - Gallstone

KW - Micelles

KW - Microscopy

KW - Nephelometry

KW - Phosphatidylcholine

KW - Phospholipids

KW - Spectrophotometry

KW - Taurocholate

KW - Vesicles

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VL - 43

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EP - 610

JO - Journal of Lipid Research

JF - Journal of Lipid Research

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