Quantitation of intracellular [Na+] in vivo by using TmDOTP5- as an NMR shift reagent and extracellular marker

Patrick M. Winter, Viswanathan Seshan, Janice D. Makos, A. Dean Sherry, Craig R. Malloy, Navin Bansal

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


A method is presented to measure the absolute concentration of intracellular Na+ ([Na+](i)) in vivo by using interleaved 23Na- and 31P-nuclear magnetic resonance (NMR) spectroscopy and TmDOTP5- as shift reagent and chemical marker of tissue extracellular space (ECS). The technique was used to determine [Na+](i) and relative ECS in livers of control rats (21 ± 3 and 0.11 ± 0.02 mM, respectively) and in rats exposed to carbon tetrachloride (103 ± 29 and 0.23 ± 0.03 mM, respectively). The NMR measurements were confirmed independently on excised tissue samples by using atomic absorption spectroscopy. The results confirm that TmDOTP5- can be used as a combined cation shift reagent and ECS marker, thereby allowing quantitation of [Na+](i) in vivo by NMR.

Original languageEnglish (US)
Pages (from-to)1806-1812
Number of pages7
JournalJournal of applied physiology
Issue number5
StatePublished - Nov 1998


  • Atomic absorption spectrometry
  • Liver
  • Nuclear magnetic resonance
  • Sodium

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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