Quantitation of the frequency of immortalization of normal human diploid fibroblasts by SV40 large T-antigen

Jerry W. Shay, Woodring E. Wright

Research output: Contribution to journalArticle

233 Scopus citations

Abstract

The mechanism by which SV40 large T-antigen immortalizes human lung fibroblasts is not yet understood, and the frequency with which immortalization occurs is unknown. Here we report detailed studies of the kinetics of immortalization. Approximately 20-50% of individual T-antigen transfected clones of IMR-90 human lung fibroblasts are able to immortalize. The failure of some clones to immortalize is consistent with the hypothesis that in some cells the aneuploidy induced by T-antigen produces extra copies of the chromosome containing a factor that must be inactivated before immortalization occurs. Within immortalization-competent clones, the frequency of immortalization is about 3×10-7. This frequency is consistent with a mutational mechanism for the inactivation of the factor that causes crisis in T-antigen expressing cells.

Original languageEnglish (US)
Pages (from-to)109-118
Number of pages10
JournalExperimental Cell Research
Volume184
Issue number1
DOIs
StatePublished - Sep 1989

ASJC Scopus subject areas

  • Cell Biology

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