TY - JOUR
T1 - Quantitative analysis for organic acids in biological samples
T2 - Batch isolation followed by gas chromatographic-mass spectrometric analysis
AU - Hoffmann, G.
AU - Aramaki, S.
AU - Blum-Hoffmann, E.
AU - Nyhan, W. L.
AU - Sweetman, L.
PY - 1989
Y1 - 1989
N2 - This new method for qualitative and quantitative determination of organic acids, aldehydes, and ketones in biological samples is effective for use with urine, plasma, and amniotic fluid, and it requires no deproteinization. Isolation by batch-wise liquid partition chromatography on silicic acid follows formation of the O-(2,3,4,5,6-pentafluorobenzyl)oximes of oxoacids, aldehydes, and ketones. The total organic acid content of the sample provides a rapid screening test for metabolic abnormality. A wide-bore, bonded-phase capillary column was used for quantitative gas chromatographic-mass spectrometric analysis, followed by automated identification and quantification. Analytical recoveries were quantitative for a wide variety of metabolites. Gas-chromatographic retention indices, discriminating ions, and control ranges in amniotic fluid, plasma, and urine of adult subjects were determined for 61 biologically important compounds.
AB - This new method for qualitative and quantitative determination of organic acids, aldehydes, and ketones in biological samples is effective for use with urine, plasma, and amniotic fluid, and it requires no deproteinization. Isolation by batch-wise liquid partition chromatography on silicic acid follows formation of the O-(2,3,4,5,6-pentafluorobenzyl)oximes of oxoacids, aldehydes, and ketones. The total organic acid content of the sample provides a rapid screening test for metabolic abnormality. A wide-bore, bonded-phase capillary column was used for quantitative gas chromatographic-mass spectrometric analysis, followed by automated identification and quantification. Analytical recoveries were quantitative for a wide variety of metabolites. Gas-chromatographic retention indices, discriminating ions, and control ranges in amniotic fluid, plasma, and urine of adult subjects were determined for 61 biologically important compounds.
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U2 - 10.1093/clinchem/35.4.587
DO - 10.1093/clinchem/35.4.587
M3 - Article
C2 - 2702744
AN - SCOPUS:0024601727
SN - 0009-9147
VL - 35
SP - 587
EP - 595
JO - Clinical chemistry
JF - Clinical chemistry
IS - 4
ER -