Quantitative protein changes in metastatic versus primary epithelial ovarian carcinoma

Stephen R. Lawson, Gerald Latter, David Scott Miller, David Goldstein, Michelle Naps, Stephen Burbeck, Nelson N H Teng, Emile Zuckerkandl

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Primary and metastatic ovarian carcinomas from six patients were obtained during primary exploratory laparotomy. Tumor cells were synthetically radiolabeled with [35S]methionine. Radiolabeled cellular proteins of the primary and metastatic cells were examined by two-dimensional polyacrylamide gel electrophoresis followed by autoradiography. Computer assisted analysis of the resultant autoradiograms revealed that the amounts of only two proteins, p35 and p36, were consistently and significantly decreased in the metastatic tumor cells. No other consistent differences in protein synthesis between primary and metastatic tumors were detected.

Original languageEnglish (US)
Pages (from-to)22-27
Number of pages6
JournalGynecologic oncology
Volume41
Issue number1
DOIs
StatePublished - Apr 1991

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

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    Lawson, S. R., Latter, G., Miller, D. S., Goldstein, D., Naps, M., Burbeck, S., Teng, N. N. H., & Zuckerkandl, E. (1991). Quantitative protein changes in metastatic versus primary epithelial ovarian carcinoma. Gynecologic oncology, 41(1), 22-27. https://doi.org/10.1016/0090-8258(91)90248-4