Quantitative telomeric overhang determination using a double-strand specific nuclease

Yong Zhao, Hirotoshi Hoshiyama, Jerry W. Shay, Woodring E. Wright

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Telomeres terminate in 3′ overhangs that function in end protection and the formation of t-loops. Determining the steps and factors involved in overhang processing is compromised by the inability to easily and accurately determine overhang size in the presence of many kilobases of double-stranded telomeric DNA. We here describe the use of a double-strand specific nuclease (DSN) that entirely digests double-stranded DNA including telomeres, leaving the overhangs intact so that they can be measured.

Original languageEnglish (US)
Article numbere14
JournalNucleic acids research
Volume36
Issue number3
DOIs
StatePublished - Feb 1 2008

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ASJC Scopus subject areas

  • Genetics

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