TY - JOUR
T1 - Quantum dots based molecular beacons for in vitro and in vivo detection of MMP-2 on tumor
AU - Li, Xin
AU - Deng, Dawei
AU - Xue, Jianpeng
AU - Qu, Lingzhi
AU - Achilefu, Samuel
AU - Gu, Yueqing
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (NSFC 81220108012, 61335007, 81371684, 81000666, 81171395 and 81328012), the Program for New Century Excellent Talents in University of the Ministry of Education of the People׳s Republic of China (NCET-12-0974), and the Major Project of the Ministry of Science and Technology of the People׳s Republic of China for new drug development ( 2009ZX-09310-004 ).
PY - 2014/11/15
Y1 - 2014/11/15
N2 - Matrix metalloproteinase-2 (MMP-2) is a protease related to tumor invasion and metastasis. It is heavily secreted by malignant tumor cells, allowing the protease to serve as an imaging biomarker of cancer. In this study, a novel sensing system based on fluorescence resonance energy transfer (FRET) from quantum dot (QD, the donor) to organic dye (the acceptor) was constructed for the in vitro and in vivo detection of matrix metalloproteinases-2 via a MMP-2-specific peptide substrate (GPLGVRGKGG). Specifically, 535. nm-emitting CdTe QD were bound to Rhodamine B (RB) through the peptide for in vitro detection of MMP-2, while 720. nm-emitting CdTeS QDs was linked to near infrared dye ICG-Der-02 (MPA) by the peptide for measurement in vivo. When these probes were exposed to MMP-2, the selective cleavage of the peptide resulted in the recovery of fluorescence from QDs. By using the produced 540QD-peptide-RB and 720QD-peptide-MPA probes, we successfully examined MMP-2 in live cells and tumor on nude mouse, respectively. Due to the tunable fluorescence of Qds, this nanosensor can be fine-tuned for a wide range of applications such as the detection of different biomarkers and early diagnosis of disease.
AB - Matrix metalloproteinase-2 (MMP-2) is a protease related to tumor invasion and metastasis. It is heavily secreted by malignant tumor cells, allowing the protease to serve as an imaging biomarker of cancer. In this study, a novel sensing system based on fluorescence resonance energy transfer (FRET) from quantum dot (QD, the donor) to organic dye (the acceptor) was constructed for the in vitro and in vivo detection of matrix metalloproteinases-2 via a MMP-2-specific peptide substrate (GPLGVRGKGG). Specifically, 535. nm-emitting CdTe QD were bound to Rhodamine B (RB) through the peptide for in vitro detection of MMP-2, while 720. nm-emitting CdTeS QDs was linked to near infrared dye ICG-Der-02 (MPA) by the peptide for measurement in vivo. When these probes were exposed to MMP-2, the selective cleavage of the peptide resulted in the recovery of fluorescence from QDs. By using the produced 540QD-peptide-RB and 720QD-peptide-MPA probes, we successfully examined MMP-2 in live cells and tumor on nude mouse, respectively. Due to the tunable fluorescence of Qds, this nanosensor can be fine-tuned for a wide range of applications such as the detection of different biomarkers and early diagnosis of disease.
KW - FRET
KW - Matrix metalloproteinase-2
KW - Near infrared imaging
KW - Proteolytic activity
KW - Quantum dots
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U2 - 10.1016/j.bios.2014.05.035
DO - 10.1016/j.bios.2014.05.035
M3 - Article
C2 - 24951921
AN - SCOPUS:84902481706
SN - 0956-5663
VL - 61
SP - 512
EP - 518
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -