Radiation-induced apoptosis in sensitive and resistant cells isolated from a mouse lymphoma

M. D. Story, D. W. Voehringer, C. G. Malone, M. L. Hobbs, R. E. Meyn

Research output: Contribution to journalArticle

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Abstract

Cells were isolated from a mouse lymphoma (LY-TH) and grown in vitro. They were susceptible to radiation-induced apoptosis after low doses with the appearance of endonucleolytically fragmented DNA 1 h after irradiation. Four hours after receiving 5 Gy, 80% of the DNA was endonucleolytically cleaved. Apoptosis induction by DNA double-strand break (dsb) formation was more effective compared with induction by single-strand break (ssb) formation. After long-term culturing, LY-TH cultures became refractory to apoptosis. Apoptosis-permissive cells (LY-as, cloned from LY-TH cells) were three times more radiosensitive than clonally expanded apoptosis-refractory cells (LY-ar). Low dose-rate irradiation and maintenance at 25°C for 5 h postirradiation was sparing in LY-ar but not LY-as cells, suggesting a repair deficiency in LY-as cells. Analysis of dsb rejoining kinetics revealed no difference in the initial phase of dsb rejoining. After 1 h, however, relatived sbs in the LY-as variant increased as endonucleolytic cleavage was initiated. Signalling for radiation-induced apoptosis in LY-as cells was independent of the DNA dsb repair pathway and appeared determined by initial events, whereas in LY-ar cells, because of an inhibition in the apoptotic pathway, survival was enhanced and modifiable by repair processes.

Original languageEnglish (US)
Pages (from-to)659-668
Number of pages10
JournalInternational Journal of Radiation Biology
Volume66
Issue number6
StatePublished - 1994

Fingerprint

apoptosis
Cell death
lymphoma
mice
Lymphoma
Radiation
Apoptosis
strands
DNA
radiation
cells
Repair
deoxyribonucleic acid
Refractory materials
Dosimetry
Double-Stranded DNA Breaks
refractories
Irradiation
induction
irradiation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Nuclear Energy and Engineering
  • Radiology Nuclear Medicine and imaging
  • Radiation
  • Radiological and Ultrasound Technology

Cite this

Radiation-induced apoptosis in sensitive and resistant cells isolated from a mouse lymphoma. / Story, M. D.; Voehringer, D. W.; Malone, C. G.; Hobbs, M. L.; Meyn, R. E.

In: International Journal of Radiation Biology, Vol. 66, No. 6, 1994, p. 659-668.

Research output: Contribution to journalArticle

Story, M. D. ; Voehringer, D. W. ; Malone, C. G. ; Hobbs, M. L. ; Meyn, R. E. / Radiation-induced apoptosis in sensitive and resistant cells isolated from a mouse lymphoma. In: International Journal of Radiation Biology. 1994 ; Vol. 66, No. 6. pp. 659-668.
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