Radiolabeled probes for imaging Alzheimer's plaques

P. V. Kulkarni, V. Arora, A. C. Roney, C. White, M. Bennett, P. P. Antich, F. J. Bonte

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Alzheimer's disease (AD) is a debilitating disease characterized by the presence of extra-cellular plaques and intra-cellular neurofibrillary tangles (NFTs) in the brain. The major protein component of these plaques is beta amyloid peptide (Aβ), a 40-42 amino acid peptide cleaved from amyloid precursor protein (APP) by β-secretase and a putative γ-secretase. We radioiodinated quinoline derivatives (clioquinol and oxine) and evaluated them as potential amyloid imaging agents based on their ability to cross the blood brain barrier (BBB) and on their selectivity to metal binding sites on amyloid plaques. The uptake of theses tracers in the brains of normal swiss-webster mice was rapid and so was the clearance. Selectivity was demonstrated by higher binding to AD brain homogenates compared to normal brain. Autoradiographic studies demonstrated the localization of the tracers in the plaque regions of the AD brain sections as well as in liver tissue with amyloidosis. Further optimization and evaluations would likely lead to development of these molecules as AD plaque imaging agents.

Original languageEnglish (US)
Pages (from-to)676-680
Number of pages5
JournalNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume241
Issue number1-4
DOIs
StatePublished - Dec 2005

Fingerprint

brain
Brain
Imaging techniques
probes
Amyloid Precursor Protein Secretases
Amyloid
Peptides
peptides
tracers
Clioquinol
selectivity
blood-brain barrier
proteins
Proteins
Oxyquinoline
theses
quinoline
clearances
Binding sites
liver

Keywords

  • Alzheimer's disease
  • Plaque imaging
  • Quinoline derivatives
  • Radiolabeled probes

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Instrumentation
  • Surfaces and Interfaces

Cite this

Radiolabeled probes for imaging Alzheimer's plaques. / Kulkarni, P. V.; Arora, V.; Roney, A. C.; White, C.; Bennett, M.; Antich, P. P.; Bonte, F. J.

In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, Vol. 241, No. 1-4, 12.2005, p. 676-680.

Research output: Contribution to journalArticle

Kulkarni, P. V. ; Arora, V. ; Roney, A. C. ; White, C. ; Bennett, M. ; Antich, P. P. ; Bonte, F. J. / Radiolabeled probes for imaging Alzheimer's plaques. In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms. 2005 ; Vol. 241, No. 1-4. pp. 676-680.
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