Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNase

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

A procedure for the removal of RNA and RNA fragments from large quantities of pBR322 plasmid DNA without the use of RNase is described. Sephacryl S-300 is employed for the separation of low-molecular-weight RNA from plasmid DNA molecules on the basis of gel filtration. The technique thus circumvents many of the dangers associated with treating plasmid DNA preparations with RNase. The procedure should be generally applicable to the purification of virtually any type of plasmid DNA isolated from a bacterial host.

Original languageEnglish (US)
Pages (from-to)34-42
Number of pages9
JournalAnalytical biochemistry
Volume113
Issue number1
DOIs
StatePublished - May 1 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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