Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNase

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A procedure for the removal of RNA and RNA fragments from large quantities of pBR322 plasmid DNA without the use of RNase is described. Sephacryl S-300 is employed for the separation of low-molecular-weight RNA from plasmid DNA molecules on the basis of gel filtration. The technique thus circumvents many of the dangers associated with treating plasmid DNA preparations with RNase. The procedure should be generally applicable to the purification of virtually any type of plasmid DNA isolated from a bacterial host.

Original languageEnglish (US)
Pages (from-to)34-42
Number of pages9
JournalAnalytical Biochemistry
Volume113
Issue number1
DOIs
StatePublished - May 1 1981

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Ribonucleases
Plasmids
RNA
DNA
Purification
Gel Chromatography
Molecular Weight
Gels
Molecular weight
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNase. / Norgard, Michael V.

In: Analytical Biochemistry, Vol. 113, No. 1, 01.05.1981, p. 34-42.

Research output: Contribution to journalArticle

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