Rapid Regeneration of Vascularized Bone by Nanofabricated Amorphous Silicon Oxynitrophosphide (SiONP) Overlays

Azhar Ilyas, Megen Velton, Ami Shah, Felipe Monte, Harry K Kim, Pranesh B. Aswath, Venu G. Varanasi

Research output: Contribution to journalArticle

Abstract

Fracture healing is a complex biological process. Severe bone loss and ischemia from traumatic fractures lead to inflammation and accumulation of damaging reactive oxygen species (ROS). Fixative devices that not only provide mechanical support but also stimulate antioxidants such as superoxide dismutase (SOD1) and influence signaling pathways for extracellular matrix (ECM) mineralization, are critical for normal healing of such fractures. In this study, we report a novel biomaterial, silicon oxynitrophosphide (SiONP) that provides sustained release of ionic silicon (Si+4) and phosphorous (P) over few weeks under physiological conditions. Anti-oxidant role of Si+4 and augmented ECM mineralization by P ions lead to enhanced osteogenesis coupled with quick revascularization for rapid bone regeneration. Plasma enhanced chemical vapor deposition (PECVD) provided a conformal, well adherent and highly reproducible surface chemistry overlaid onto nanofabricated bioinspired surfaces. The Nitrogen to P and O content ratio was observed to change the dissolution rate and the release kinetics of the overlaid film. The SiONP films with optimal release kinetics promoted anti-oxidant expression via enhanced SOD1, which downstream upregulated other osteogenic markers with MC3T3-E1 cells. These surfaces also promoted angiogenesis evident by formation of thicker tubules by Human umbilical vein endothelial cells (HUVEC). In-vivo evaluation using a rat critical-sized calvarial defect model showed rapid bone-regeneration for these nanofabricated biomaterials as compared to control groups, and opens new horizon for future clinical trials of new antioxidant materials on biomedical devices that can reduce healing time, lower medical care cost, and increase the quality of newly formed bone in critical size defects.

Original languageEnglish (US)
Pages (from-to)1241-1255
Number of pages15
JournalJournal of biomedical nanotechnology
Volume15
Issue number6
DOIs
StatePublished - Jun 1 2019

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Bone Regeneration
Silicon
Amorphous silicon
Bone
Fracture Healing
Biocompatible Materials
Oxidants
Extracellular Matrix
Antioxidants
Biomaterials
Biological Phenomena
Bone and Bones
Equipment and Supplies
Fixatives
Human Umbilical Vein Endothelial Cells
Osteogenesis
Health Care Costs
Defects
Superoxide Dismutase
Kinetics

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

Rapid Regeneration of Vascularized Bone by Nanofabricated Amorphous Silicon Oxynitrophosphide (SiONP) Overlays. / Ilyas, Azhar; Velton, Megen; Shah, Ami; Monte, Felipe; Kim, Harry K; Aswath, Pranesh B.; Varanasi, Venu G.

In: Journal of biomedical nanotechnology, Vol. 15, No. 6, 01.06.2019, p. 1241-1255.

Research output: Contribution to journalArticle

Ilyas, Azhar ; Velton, Megen ; Shah, Ami ; Monte, Felipe ; Kim, Harry K ; Aswath, Pranesh B. ; Varanasi, Venu G. / Rapid Regeneration of Vascularized Bone by Nanofabricated Amorphous Silicon Oxynitrophosphide (SiONP) Overlays. In: Journal of biomedical nanotechnology. 2019 ; Vol. 15, No. 6. pp. 1241-1255.
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