Rapid ultrasensitive detection platform for antimicrobial susceptibility testing

Mehmet F. Cansizoglu, Yusuf Talha Tamer, Michael Farid, Andrew Koh, Erdal Toprak

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Rapid detection and phenotyping of pathogenic microbes is critical for administration of effective antibiotic therapies and for impeding the spread of antibiotic resistance. Here, we present a novel platform, rapid ultrasensitive detector (RUSD), that utilizes the high reflectance coefficient at high incidence angles when light travels from low- to high-refractive-index media. RUSD leverages a principle that does not require complex manufacturing, labeling, or processing steps. Utilizing RUSD, we can detect extremely low cell densities (optical density [OD] ≥ 5 × 10-7) that correspond to approximately 20 bacterial cells or a single fungal cell in the detection volume, which is nearly 4 orders of magnitude more sensitive than standard OD methods. RUSD can measure minimum inhibitory concentrations (MICs) of commonly used antibiotics against gram-negative and gram-positive bacteria, including Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli, within 2 to 4 h. Here, we demonstrate that antibiotic susceptibility tests for several pathogens can rapidly be performed with RUSD using both small inoculum sizes (500 cells/mL) and larger inoculum sizes (5 × 105 cells/mL) used in standard antibiotic susceptibility tests. We anticipate that the RUSD system will be particularly useful for the cases in which antibiotic susceptibility tests have to be done with a limited number of bacterial cells that are available. Its compatibility with standard antibiotic susceptibility tests, simplicity, and low cost can make RUSD a viable and rapidly deployed diagnostic tool.

Original languageEnglish (US)
Pages (from-to)e3000291
JournalPLoS biology
Volume17
Issue number5
DOIs
StatePublished - May 1 2019

Fingerprint

detectors
anti-infective agents
Anti-Bacterial Agents
Detectors
antibiotics
Testing
testing
Density (optical)
cells
absorbance
Cell Count
inoculum
Refractometry
Gram-Positive Bacteria
Microbial Sensitivity Tests
Microbial Drug Resistance
refractive index
Cell Size
Pseudomonas aeruginosa
Pathogens

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Rapid ultrasensitive detection platform for antimicrobial susceptibility testing. / Cansizoglu, Mehmet F.; Tamer, Yusuf Talha; Farid, Michael; Koh, Andrew; Toprak, Erdal.

In: PLoS biology, Vol. 17, No. 5, 01.05.2019, p. e3000291.

Research output: Contribution to journalArticle

Cansizoglu, Mehmet F. ; Tamer, Yusuf Talha ; Farid, Michael ; Koh, Andrew ; Toprak, Erdal. / Rapid ultrasensitive detection platform for antimicrobial susceptibility testing. In: PLoS biology. 2019 ; Vol. 17, No. 5. pp. e3000291.
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