Rat-brain Na,K-ATPase beta-chain gene: primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells.

R. W. Mercer, J. W. Schneider, A. Savitz, J. Emanuel, E. J. Benz, R. Levenson

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

We deduced the complete amino acid sequence of the rat brain Na,K-ATPase beta-subunit from cDNA. The rat brain beta-subunit exhibits a high degree of primary sequence and secondary structural homology with the human and Torpedo beta-subunit polypeptides. Analysis of rat tissue RNA reveals that the beta-subunit gene encodes four separate mRNA species which are expressed in a tissue-specific fashion. In ouabain-resistant HeLa C+ cells, beta-subunit DNA sequences are amplified (approximately 20-fold) and beta-subunit mRNAs are overproduced relative to levels in parental HeLa cells. These results suggest that the beta-subunit plays an important role in Na,K-ATPase structure-function and in the mechanism underlying cellular resistance to the cardiac glycosides.

Original languageEnglish (US)
Pages (from-to)3884-3890
Number of pages7
JournalMolecular and cellular biology
Volume6
Issue number11
DOIs
StatePublished - Nov 1986

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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