Reagent-and separation-free measurements of urine creatinine concentration using stamping surface enhanced Raman scattering (S-SERS)

Ming Li, Yong Du, Fusheng Zhao, Jianbo Zeng, Chandra Mohan, Wei Chuan Shih

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We report a novel reagent-and separation-free method for urine creatinine concentration measurement using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) plasmonic substrates, a label-free, multiplexed molecular sensing and imaging technique recently developed by us. The performance of this new technology is evaluated by the detection and quantification of creatinine spiked in three different liquids: creatinine in water, mixture of creatinine and urea in water, and creatinine in artificial urine within physiologically relevant concentration ranges. Moreover, the potential application of our method is demonstrated by creatinine concentration measurements in urine samples collected from a mouse model of nephritis. The limit of detection of creatinine was 13.2 nM (0.15 μg/dl) and 0.68 mg/dl in water and urine, respectively. Our method would provide an alternative tool for rapid, costeffective, and reliable urine analysis for non-invasive diagnosis and monitoring of renal function.

Original languageEnglish (US)
Pages (from-to)849-858
Number of pages10
JournalBiomedical Optics Express
Volume6
Issue number3
DOIs
StatePublished - 2015

Fingerprint

creatinine
stamping
urine
Raman Spectrum Analysis
reagents
Creatinine
Urine
Raman spectra
Water
nephritis
renal function
water
Molecular Imaging
Nephritis
ureas
imaging techniques
Gold
mice
Urea
Limit of Detection

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

Reagent-and separation-free measurements of urine creatinine concentration using stamping surface enhanced Raman scattering (S-SERS). / Li, Ming; Du, Yong; Zhao, Fusheng; Zeng, Jianbo; Mohan, Chandra; Shih, Wei Chuan.

In: Biomedical Optics Express, Vol. 6, No. 3, 2015, p. 849-858.

Research output: Contribution to journalArticle

Li, Ming ; Du, Yong ; Zhao, Fusheng ; Zeng, Jianbo ; Mohan, Chandra ; Shih, Wei Chuan. / Reagent-and separation-free measurements of urine creatinine concentration using stamping surface enhanced Raman scattering (S-SERS). In: Biomedical Optics Express. 2015 ; Vol. 6, No. 3. pp. 849-858.
@article{2453ff80c3594642b5ba8d8fb726f2cd,
title = "Reagent-and separation-free measurements of urine creatinine concentration using stamping surface enhanced Raman scattering (S-SERS)",
abstract = "We report a novel reagent-and separation-free method for urine creatinine concentration measurement using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) plasmonic substrates, a label-free, multiplexed molecular sensing and imaging technique recently developed by us. The performance of this new technology is evaluated by the detection and quantification of creatinine spiked in three different liquids: creatinine in water, mixture of creatinine and urea in water, and creatinine in artificial urine within physiologically relevant concentration ranges. Moreover, the potential application of our method is demonstrated by creatinine concentration measurements in urine samples collected from a mouse model of nephritis. The limit of detection of creatinine was 13.2 nM (0.15 μg/dl) and 0.68 mg/dl in water and urine, respectively. Our method would provide an alternative tool for rapid, costeffective, and reliable urine analysis for non-invasive diagnosis and monitoring of renal function.",
author = "Ming Li and Yong Du and Fusheng Zhao and Jianbo Zeng and Chandra Mohan and Shih, {Wei Chuan}",
year = "2015",
doi = "10.1364/BOE.6.000849",
language = "English (US)",
volume = "6",
pages = "849--858",
journal = "Biomedical Optics Express",
issn = "2156-7085",
publisher = "The Optical Society",
number = "3",

}

TY - JOUR

T1 - Reagent-and separation-free measurements of urine creatinine concentration using stamping surface enhanced Raman scattering (S-SERS)

AU - Li, Ming

AU - Du, Yong

AU - Zhao, Fusheng

AU - Zeng, Jianbo

AU - Mohan, Chandra

AU - Shih, Wei Chuan

PY - 2015

Y1 - 2015

N2 - We report a novel reagent-and separation-free method for urine creatinine concentration measurement using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) plasmonic substrates, a label-free, multiplexed molecular sensing and imaging technique recently developed by us. The performance of this new technology is evaluated by the detection and quantification of creatinine spiked in three different liquids: creatinine in water, mixture of creatinine and urea in water, and creatinine in artificial urine within physiologically relevant concentration ranges. Moreover, the potential application of our method is demonstrated by creatinine concentration measurements in urine samples collected from a mouse model of nephritis. The limit of detection of creatinine was 13.2 nM (0.15 μg/dl) and 0.68 mg/dl in water and urine, respectively. Our method would provide an alternative tool for rapid, costeffective, and reliable urine analysis for non-invasive diagnosis and monitoring of renal function.

AB - We report a novel reagent-and separation-free method for urine creatinine concentration measurement using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) plasmonic substrates, a label-free, multiplexed molecular sensing and imaging technique recently developed by us. The performance of this new technology is evaluated by the detection and quantification of creatinine spiked in three different liquids: creatinine in water, mixture of creatinine and urea in water, and creatinine in artificial urine within physiologically relevant concentration ranges. Moreover, the potential application of our method is demonstrated by creatinine concentration measurements in urine samples collected from a mouse model of nephritis. The limit of detection of creatinine was 13.2 nM (0.15 μg/dl) and 0.68 mg/dl in water and urine, respectively. Our method would provide an alternative tool for rapid, costeffective, and reliable urine analysis for non-invasive diagnosis and monitoring of renal function.

UR - http://www.scopus.com/inward/record.url?scp=84942365817&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84942365817&partnerID=8YFLogxK

U2 - 10.1364/BOE.6.000849

DO - 10.1364/BOE.6.000849

M3 - Article

C2 - 25798309

AN - SCOPUS:84942365817

VL - 6

SP - 849

EP - 858

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 3

ER -