Recombinant immunoblot and polymerase chain reaction testing in volunteer whole blood donors screened by a multi-antigen assay for hepatitis C virus antibodies

M. H. Sayers, D. R. Gretch

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The purpose of this study was to compare the results of supplementary testing of volunteer whole blood donors who had been screened by the first hepatitis C virus antibody assay licensed in the United States with results from donors screened by a newer, more sensitive, multi-antigen assay. In contrast to the earlier assay, the multi-antigen assay incorporates a recombinant hepatitis C virus antigen, c22-3, which is encoded by a structural region of the viral genome. Supplementary testing included a second generation recombinant immunoblot assay and a highly sensitive polymerase chain reaction assay for evidence of hepatitis C virus genomic RNA. A comparison of supplementary test results reveals a higher percentage of donors screened by the newer assay to be indeterminate on recombinant immunoblot (34.4% vs. 6.4%, p<0.05). Furthermore, polymerase chain reaction testing of donors with indeterminate blot results shows that 14 percent have evidence of viral RNA. For this reason, counseling of donors with indeterminate patterns on immunoblot must include informing them of the possibility that they are infected.

Original languageEnglish (US)
Pages (from-to)809-813
Number of pages5
JournalTransfusion
Volume33
Issue number10
StatePublished - 1993

Fingerprint

Hepatitis C Antibodies
Blood Donors
Volunteers
Tissue Donors
Antigens
Polymerase Chain Reaction
Hepacivirus
Hepatitis C Antigens
Viral Genome
Viral RNA
Counseling
RNA

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

@article{629d2c75675040c99039c896185e7ca8,
title = "Recombinant immunoblot and polymerase chain reaction testing in volunteer whole blood donors screened by a multi-antigen assay for hepatitis C virus antibodies",
abstract = "The purpose of this study was to compare the results of supplementary testing of volunteer whole blood donors who had been screened by the first hepatitis C virus antibody assay licensed in the United States with results from donors screened by a newer, more sensitive, multi-antigen assay. In contrast to the earlier assay, the multi-antigen assay incorporates a recombinant hepatitis C virus antigen, c22-3, which is encoded by a structural region of the viral genome. Supplementary testing included a second generation recombinant immunoblot assay and a highly sensitive polymerase chain reaction assay for evidence of hepatitis C virus genomic RNA. A comparison of supplementary test results reveals a higher percentage of donors screened by the newer assay to be indeterminate on recombinant immunoblot (34.4{\%} vs. 6.4{\%}, p<0.05). Furthermore, polymerase chain reaction testing of donors with indeterminate blot results shows that 14 percent have evidence of viral RNA. For this reason, counseling of donors with indeterminate patterns on immunoblot must include informing them of the possibility that they are infected.",
author = "Sayers, {M. H.} and Gretch, {D. R.}",
year = "1993",
language = "English (US)",
volume = "33",
pages = "809--813",
journal = "Transfusion",
issn = "0041-1132",
publisher = "Wiley-Blackwell",
number = "10",

}

TY - JOUR

T1 - Recombinant immunoblot and polymerase chain reaction testing in volunteer whole blood donors screened by a multi-antigen assay for hepatitis C virus antibodies

AU - Sayers, M. H.

AU - Gretch, D. R.

PY - 1993

Y1 - 1993

N2 - The purpose of this study was to compare the results of supplementary testing of volunteer whole blood donors who had been screened by the first hepatitis C virus antibody assay licensed in the United States with results from donors screened by a newer, more sensitive, multi-antigen assay. In contrast to the earlier assay, the multi-antigen assay incorporates a recombinant hepatitis C virus antigen, c22-3, which is encoded by a structural region of the viral genome. Supplementary testing included a second generation recombinant immunoblot assay and a highly sensitive polymerase chain reaction assay for evidence of hepatitis C virus genomic RNA. A comparison of supplementary test results reveals a higher percentage of donors screened by the newer assay to be indeterminate on recombinant immunoblot (34.4% vs. 6.4%, p<0.05). Furthermore, polymerase chain reaction testing of donors with indeterminate blot results shows that 14 percent have evidence of viral RNA. For this reason, counseling of donors with indeterminate patterns on immunoblot must include informing them of the possibility that they are infected.

AB - The purpose of this study was to compare the results of supplementary testing of volunteer whole blood donors who had been screened by the first hepatitis C virus antibody assay licensed in the United States with results from donors screened by a newer, more sensitive, multi-antigen assay. In contrast to the earlier assay, the multi-antigen assay incorporates a recombinant hepatitis C virus antigen, c22-3, which is encoded by a structural region of the viral genome. Supplementary testing included a second generation recombinant immunoblot assay and a highly sensitive polymerase chain reaction assay for evidence of hepatitis C virus genomic RNA. A comparison of supplementary test results reveals a higher percentage of donors screened by the newer assay to be indeterminate on recombinant immunoblot (34.4% vs. 6.4%, p<0.05). Furthermore, polymerase chain reaction testing of donors with indeterminate blot results shows that 14 percent have evidence of viral RNA. For this reason, counseling of donors with indeterminate patterns on immunoblot must include informing them of the possibility that they are infected.

UR - http://www.scopus.com/inward/record.url?scp=0027382951&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027382951&partnerID=8YFLogxK

M3 - Article

VL - 33

SP - 809

EP - 813

JO - Transfusion

JF - Transfusion

SN - 0041-1132

IS - 10

ER -