Recombinant porcine prorelaxin produced in Chinese hamster ovary cells is biologically active

Ann L. Vu, Carla B. Green, Katherine F. Roby, Michael J. Soares, David T W Fei, Anthony B. Chen, Simon C M Kwok

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Although prorelaxin has a similar structure as proinsulin, the posttranslational processing of prorelaxin seems to be quite different from that of proinsulin. There are no pairs of basic residues flanking the relaxin moiety in most prorelaxins studied so far. Instead, the prorelaxins of many species contains a tetrabasic sequence (Arg-Lys-Lys-Arg) between the connecting peptide and the A-chain. This is the recognition sequence of furin. In order to study this possible processing by furin, we express the recombinant porcine prorelaxin in Chinese hamster ovary cells. The expected 19 kDa recombinant porcine prorelaxin was found to be constitutively secreted into the medium at a level of approximately 250 ng/ml. No conversion of the 19 kDa prorelaxin into the 6 kDa relaxin was observed. Unlike most prohormones which are biologically inactive, the recombinant prorelaxin was found to be biologically active in an in vitro bioassay.

Original languageEnglish (US)
Pages (from-to)1055-1061
Number of pages7
JournalLife Sciences
Volume52
Issue number12
DOIs
StatePublished - 1993

Fingerprint

Cricetulus
Ovary
Swine
Cells
Furin
Relaxin
Proinsulin
Bioassay
C-Peptide
Processing
Biological Assay
prorelaxin

ASJC Scopus subject areas

  • Pharmacology

Cite this

Vu, A. L., Green, C. B., Roby, K. F., Soares, M. J., Fei, D. T. W., Chen, A. B., & Kwok, S. C. M. (1993). Recombinant porcine prorelaxin produced in Chinese hamster ovary cells is biologically active. Life Sciences, 52(12), 1055-1061. https://doi.org/10.1016/0024-3205(93)90198-C

Recombinant porcine prorelaxin produced in Chinese hamster ovary cells is biologically active. / Vu, Ann L.; Green, Carla B.; Roby, Katherine F.; Soares, Michael J.; Fei, David T W; Chen, Anthony B.; Kwok, Simon C M.

In: Life Sciences, Vol. 52, No. 12, 1993, p. 1055-1061.

Research output: Contribution to journalArticle

Vu, Ann L. ; Green, Carla B. ; Roby, Katherine F. ; Soares, Michael J. ; Fei, David T W ; Chen, Anthony B. ; Kwok, Simon C M. / Recombinant porcine prorelaxin produced in Chinese hamster ovary cells is biologically active. In: Life Sciences. 1993 ; Vol. 52, No. 12. pp. 1055-1061.
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AU - Chen, Anthony B.

AU - Kwok, Simon C M

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