Reconstitution of modified chromatin templates for in vitro functional assays

Miyong Yun, Chun Ruan, Jae Wan Huh, Bing Li

Research output: Chapter in Book/Report/Conference proceedingChapter

15 Citations (Scopus)

Abstract

To study the functions of histone modifications in the context of chromatin, it is necessary to be able to prepare nucleosomal templates that carry specific posttranslational modifications in a defined biochemical system. Here, we describe two sets of protocols for reconstituting designer nucleosomes that contain specifically modified histones. The resulting nucleosomes are suitable for electromobility shift assays, chromatin remodeling assays, and other functional and structural studies.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages237-253
Number of pages17
Volume833
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume833
ISSN (Print)10643745

Fingerprint

Nucleosomes
Chromatin
Histone Code
Chromatin Assembly and Disassembly
Post Translational Protein Processing
Histones
In Vitro Techniques

Keywords

  • Chromatin
  • Histone modification
  • In vitro
  • Nucleosome
  • Reconstitution

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yun, M., Ruan, C., Huh, J. W., & Li, B. (2012). Reconstitution of modified chromatin templates for in vitro functional assays. In Methods in Molecular Biology (Vol. 833, pp. 237-253). (Methods in Molecular Biology; Vol. 833). https://doi.org/10.1007/978-1-61779-477-3_15

Reconstitution of modified chromatin templates for in vitro functional assays. / Yun, Miyong; Ruan, Chun; Huh, Jae Wan; Li, Bing.

Methods in Molecular Biology. Vol. 833 2012. p. 237-253 (Methods in Molecular Biology; Vol. 833).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yun, M, Ruan, C, Huh, JW & Li, B 2012, Reconstitution of modified chromatin templates for in vitro functional assays. in Methods in Molecular Biology. vol. 833, Methods in Molecular Biology, vol. 833, pp. 237-253. https://doi.org/10.1007/978-1-61779-477-3_15
Yun M, Ruan C, Huh JW, Li B. Reconstitution of modified chromatin templates for in vitro functional assays. In Methods in Molecular Biology. Vol. 833. 2012. p. 237-253. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-477-3_15
Yun, Miyong ; Ruan, Chun ; Huh, Jae Wan ; Li, Bing. / Reconstitution of modified chromatin templates for in vitro functional assays. Methods in Molecular Biology. Vol. 833 2012. pp. 237-253 (Methods in Molecular Biology).
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