Reconstitution of modified chromatin templates for in vitro functional assays

Miyong Yun, Chun Ruan, Jae Wan Huh, Bing Li

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Scopus citations

Abstract

To study the functions of histone modifications in the context of chromatin, it is necessary to be able to prepare nucleosomal templates that carry specific posttranslational modifications in a defined biochemical system. Here, we describe two sets of protocols for reconstituting designer nucleosomes that contain specifically modified histones. The resulting nucleosomes are suitable for electromobility shift assays, chromatin remodeling assays, and other functional and structural studies.

Original languageEnglish (US)
Title of host publicationChromatin Remodeling
Subtitle of host publicationMethods and Protocols
EditorsRandall H. Morse
Pages237-253
Number of pages17
DOIs
StatePublished - Jan 20 2012

Publication series

NameMethods in Molecular Biology
Volume833
ISSN (Print)1064-3745

Keywords

  • Chromatin
  • Histone modification
  • In vitro
  • Nucleosome
  • Reconstitution

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Yun, M., Ruan, C., Huh, J. W., & Li, B. (2012). Reconstitution of modified chromatin templates for in vitro functional assays. In R. H. Morse (Ed.), Chromatin Remodeling: Methods and Protocols (pp. 237-253). (Methods in Molecular Biology; Vol. 833). https://doi.org/10.1007/978-1-61779-477-3_15