Reconstruction of mammalian cells from nuclear and cytoplasmic components separated by treatment with cytochalasin B

G. Veomett, D. M. Prescott, J. Shay, K. R. Porter

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Abstract

Mouse L929 cells were separated into enucleated cytoplasmic components (cytoplasts) and nucleated subcellular fractions (karyoplasts) in the presence of cytochalasin B. Karyoplasts from cells containing tritiated nuclei were fused, using inactivated Sendai virus, to cytoplasts from cells containing large (1.0 μm diameter) latex spheres in the cytoplasm. Mononucleated cells containing radioactive nuclei and large latex spheres in the cytoplasm were observed among the products of the fusion reaction. Some of these cells were in mitotic configurations. The results indicate that cells capable of undergoing mitosis can be reconstructed from the products of cellular enucleation in the presence of cytochalasin B.

Original languageEnglish (US)
Pages (from-to)1999-2002
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume71
Issue number5
Publication statusPublished - 1974

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ASJC Scopus subject areas

  • General
  • Genetics

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