Recovery time of corneal epithelial proliferation in the rabbit following rigid gas-permeable extended contact-lens wear

Patrick M. Ladage, Kazuaki Yamamoto, David H. Ren, James V. Jester, Walter M Petroll, Jan P G Bergmanson, Harrison D Cavanagh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Purpose. To determine recovery time needed for the corneal epithelium to return to a normal proliferation rate following 24-hour rigid gas-permeable (RGP) lens wear. Methods. An RGP lens (Dk/t = 10) was fitted at 9:00 AM on one randomly chosen eye of each rabbit (N = 16) and removed 24 hours later while the other eye served as a control. Following contact lens removal, the rabbits were injected at four different time intervals (1,2, 4, and 7 days) with 5-bromo-deoxyuridine (BrdU) at 9:00 AM to label dividing corneal epithelial cells. All animals were sacrificed 24 hours after BrdU injection, and corneas were stained for BrdU. A series of continuously adjacent digital images of the whole-mount epithelium was collected from superior limbus to central cornea, and all BrdU-labeled epithelial cell pairs on each digitized image were counted. Results. On day 1 following lens removal, the number of BrdU-labeled corneal epithelium cells was reduced by 50% centrally (C) and 36% peripherally (P) (P < 0.001) compared with control eyes. At day 2, there was no statistically significant difference between control and experimental corneas (P = 0.675). At day 4, a marked significant increase in BrdU-labeled cells was noted, averaging 83% (C) and 96% (P) (P < 0.001). At day 7, BrdU-labeling returned to control values, although it was still slightly elevated (14% [C] and 5% [P], P = 0.045). Conclusions. The corneal epithelium in the rabbit required at least 1 full week to recover to its stable baseline proliferation rate following only 24 hours of RGP lens wear. Interestingly, hyperproliferation was noted within the limbal, peripheral, and mid-peripheral corneal epithelium 4 days after lens removal.

Original languageEnglish (US)
Pages (from-to)61-64
Number of pages4
JournalEye and Contact Lens
Volume29
Issue number2
DOIs
StatePublished - Apr 2003

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Extended-Wear Contact Lenses
Deoxyuridine
Gases
Corneal Epithelium
Rabbits
Lenses
Cornea
Epithelial Cells
Contact Lenses
Epithelium
Injections

Keywords

  • Contact lens
  • Corneal epithelium
  • Extended wear
  • Proliferation
  • Recovery

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Recovery time of corneal epithelial proliferation in the rabbit following rigid gas-permeable extended contact-lens wear. / Ladage, Patrick M.; Yamamoto, Kazuaki; Ren, David H.; Jester, James V.; Petroll, Walter M; Bergmanson, Jan P G; Cavanagh, Harrison D.

In: Eye and Contact Lens, Vol. 29, No. 2, 04.2003, p. 61-64.

Research output: Contribution to journalArticle

Ladage, Patrick M. ; Yamamoto, Kazuaki ; Ren, David H. ; Jester, James V. ; Petroll, Walter M ; Bergmanson, Jan P G ; Cavanagh, Harrison D. / Recovery time of corneal epithelial proliferation in the rabbit following rigid gas-permeable extended contact-lens wear. In: Eye and Contact Lens. 2003 ; Vol. 29, No. 2. pp. 61-64.
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abstract = "Purpose. To determine recovery time needed for the corneal epithelium to return to a normal proliferation rate following 24-hour rigid gas-permeable (RGP) lens wear. Methods. An RGP lens (Dk/t = 10) was fitted at 9:00 AM on one randomly chosen eye of each rabbit (N = 16) and removed 24 hours later while the other eye served as a control. Following contact lens removal, the rabbits were injected at four different time intervals (1,2, 4, and 7 days) with 5-bromo-deoxyuridine (BrdU) at 9:00 AM to label dividing corneal epithelial cells. All animals were sacrificed 24 hours after BrdU injection, and corneas were stained for BrdU. A series of continuously adjacent digital images of the whole-mount epithelium was collected from superior limbus to central cornea, and all BrdU-labeled epithelial cell pairs on each digitized image were counted. Results. On day 1 following lens removal, the number of BrdU-labeled corneal epithelium cells was reduced by 50{\%} centrally (C) and 36{\%} peripherally (P) (P < 0.001) compared with control eyes. At day 2, there was no statistically significant difference between control and experimental corneas (P = 0.675). At day 4, a marked significant increase in BrdU-labeled cells was noted, averaging 83{\%} (C) and 96{\%} (P) (P < 0.001). At day 7, BrdU-labeling returned to control values, although it was still slightly elevated (14{\%} [C] and 5{\%} [P], P = 0.045). Conclusions. The corneal epithelium in the rabbit required at least 1 full week to recover to its stable baseline proliferation rate following only 24 hours of RGP lens wear. Interestingly, hyperproliferation was noted within the limbal, peripheral, and mid-peripheral corneal epithelium 4 days after lens removal.",
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T1 - Recovery time of corneal epithelial proliferation in the rabbit following rigid gas-permeable extended contact-lens wear

AU - Ladage, Patrick M.

AU - Yamamoto, Kazuaki

AU - Ren, David H.

AU - Jester, James V.

AU - Petroll, Walter M

AU - Bergmanson, Jan P G

AU - Cavanagh, Harrison D

PY - 2003/4

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N2 - Purpose. To determine recovery time needed for the corneal epithelium to return to a normal proliferation rate following 24-hour rigid gas-permeable (RGP) lens wear. Methods. An RGP lens (Dk/t = 10) was fitted at 9:00 AM on one randomly chosen eye of each rabbit (N = 16) and removed 24 hours later while the other eye served as a control. Following contact lens removal, the rabbits were injected at four different time intervals (1,2, 4, and 7 days) with 5-bromo-deoxyuridine (BrdU) at 9:00 AM to label dividing corneal epithelial cells. All animals were sacrificed 24 hours after BrdU injection, and corneas were stained for BrdU. A series of continuously adjacent digital images of the whole-mount epithelium was collected from superior limbus to central cornea, and all BrdU-labeled epithelial cell pairs on each digitized image were counted. Results. On day 1 following lens removal, the number of BrdU-labeled corneal epithelium cells was reduced by 50% centrally (C) and 36% peripherally (P) (P < 0.001) compared with control eyes. At day 2, there was no statistically significant difference between control and experimental corneas (P = 0.675). At day 4, a marked significant increase in BrdU-labeled cells was noted, averaging 83% (C) and 96% (P) (P < 0.001). At day 7, BrdU-labeling returned to control values, although it was still slightly elevated (14% [C] and 5% [P], P = 0.045). Conclusions. The corneal epithelium in the rabbit required at least 1 full week to recover to its stable baseline proliferation rate following only 24 hours of RGP lens wear. Interestingly, hyperproliferation was noted within the limbal, peripheral, and mid-peripheral corneal epithelium 4 days after lens removal.

AB - Purpose. To determine recovery time needed for the corneal epithelium to return to a normal proliferation rate following 24-hour rigid gas-permeable (RGP) lens wear. Methods. An RGP lens (Dk/t = 10) was fitted at 9:00 AM on one randomly chosen eye of each rabbit (N = 16) and removed 24 hours later while the other eye served as a control. Following contact lens removal, the rabbits were injected at four different time intervals (1,2, 4, and 7 days) with 5-bromo-deoxyuridine (BrdU) at 9:00 AM to label dividing corneal epithelial cells. All animals were sacrificed 24 hours after BrdU injection, and corneas were stained for BrdU. A series of continuously adjacent digital images of the whole-mount epithelium was collected from superior limbus to central cornea, and all BrdU-labeled epithelial cell pairs on each digitized image were counted. Results. On day 1 following lens removal, the number of BrdU-labeled corneal epithelium cells was reduced by 50% centrally (C) and 36% peripherally (P) (P < 0.001) compared with control eyes. At day 2, there was no statistically significant difference between control and experimental corneas (P = 0.675). At day 4, a marked significant increase in BrdU-labeled cells was noted, averaging 83% (C) and 96% (P) (P < 0.001). At day 7, BrdU-labeling returned to control values, although it was still slightly elevated (14% [C] and 5% [P], P = 0.045). Conclusions. The corneal epithelium in the rabbit required at least 1 full week to recover to its stable baseline proliferation rate following only 24 hours of RGP lens wear. Interestingly, hyperproliferation was noted within the limbal, peripheral, and mid-peripheral corneal epithelium 4 days after lens removal.

KW - Contact lens

KW - Corneal epithelium

KW - Extended wear

KW - Proliferation

KW - Recovery

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