Regulated membrane protein entry into flagella is facilitated by cytoplasmic microtubules and does not require IFT

Olivier Belzile, Carmen I. Hernandez-Lara, Qian Wang, William J. Snell

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The membrane protein composition of the primary cilium, a key sensory organelle, is dynamically regulated during cilium-generated signaling [1, 2]. During ciliogenesis, ciliary membrane proteins, along with structural and signaling proteins, are carried through the multicomponent, intensely studied ciliary diffusion barrier at the base of the organelle [3-8] by intraflagellar transport (IFT) [9-18]. A favored model is that signaling-triggered accumulation of previously excluded membrane proteins in fully formed cilia [19-21] also requires IFT, but direct evidence is lacking. Here, in studies of regulated entry of a membrane protein into the flagellum of Chlamydomonas, we show that cells use an IFT-independent mechanism to breach the diffusion barrier at the flagellar base. In resting cells, a flagellar signaling component [22], the integral membrane polypeptide SAG1-C65, is uniformly distributed over the plasma membrane and excluded from the flagellar membrane. Flagellar adhesion-induced signaling triggers rapid, striking redistribution of the protein to the apical ends of the cells concomitantly with entry into the flagella. Protein polarization and flagellar enrichment are facilitated by cytoplasmic microtubules. Using a conditional anterograde IFT mutant, we demonstrate that the IFT machinery is not required for regulated SAG1-C65 entry into flagella. Thus, integral membrane proteins can negotiate passage through the ciliary diffusion barrier without the need for a motor.

Original languageEnglish (US)
Pages (from-to)1460-1465
Number of pages6
JournalCurrent Biology
Volume23
Issue number15
DOIs
StatePublished - Aug 5 2013

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Flagella
flagellum
Microtubules
membrane proteins
microtubules
Membrane Proteins
Diffusion barriers
Cilia
cilia
Organelles
organelles
Membranes
Chlamydomonas
Proteins
proteins
protein composition
cells
Cell membranes
Machinery
adhesion

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Regulated membrane protein entry into flagella is facilitated by cytoplasmic microtubules and does not require IFT. / Belzile, Olivier; Hernandez-Lara, Carmen I.; Wang, Qian; Snell, William J.

In: Current Biology, Vol. 23, No. 15, 05.08.2013, p. 1460-1465.

Research output: Contribution to journalArticle

Belzile, Olivier ; Hernandez-Lara, Carmen I. ; Wang, Qian ; Snell, William J. / Regulated membrane protein entry into flagella is facilitated by cytoplasmic microtubules and does not require IFT. In: Current Biology. 2013 ; Vol. 23, No. 15. pp. 1460-1465.
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