Regulating Cell Surface Glycosylation by Small Molecule Control of Enzyme Localization

Jennifer J. Kohler, Carolyn R. Bertozzi

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Cell surface carbohydrates mediate interactions between the cell and its environment. Glycosyltransferases responsible for synthesis of cell surface oligosaccharides are therefore essential administrators of cellular communication. These enzymes often comprise large families. Redundacy of related family members and embryonic lethality both complicate genetic methods for deconvoluting functions of glycosyltransferases. We report a chemical method in which the activity of an individual glycosyltransferase is controlled by a small molecule. The approach exploits the requirement of Golgi localization, a common feature of glycosyltransferase superfamily members. In our approach, the glycosyltransferase is separated into two domains, one that determines localization and one responsible for catalysis. Control of enzyme activity is achieved using a small molecule to regulate association of the two domains. We used this method to regulate production of sialyl Lewis x by α1,3-fucosyltransferase VII in living cells.

Original languageEnglish (US)
Pages (from-to)1303-1311
Number of pages9
JournalChemistry and Biology
Volume10
Issue number12
DOIs
StatePublished - Dec 2003

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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