Regulation and the role of Cu,Zn-containing superoxide dismutase in cell cycle progression of Schizosaccharomyces pombe

Joon Lee, Eun Soo Kwon, Dong Wook Kim, Joonseok Cha, Jung Hye Roe

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Regulation and the role of the sod1+ gene encoding CuZnSOD were investigated in fission yeast Schizosaccharomyces pombe. The amount of sod1+ mRNA decreased in the stationary phase, consistent with the decrease in enzyme activity. The transcript increased by treatment with oxidants such as H2O2 and menadione (MD). Induction by H2O2 was rapid and transient, being dependent on Wis1-Spc1-Atf1 pathway of signal transduction, whereas induction by MD was slow and sustained longer, being independent of Wis1 pathway. Wis1 and Spc1 also turned out to down-regulate sod1+ gene at the stationary phase. Tetrad analysis following sod1+ gene disruption revealed that the sod1Δ cells were not viable, even on rich media. Repression of the sod1+ gene expression by thiamine through nmt1 promoter resulted in the arrest of cell cycle progression following S phase, possibly between G2 and cytokinesis. The current and previous observations that the viability of Schizosaccharomyces pombe cells, unlike Saccharomyces cerevisiae, critically depends on the action of oxidative defense enzymes in the cytosol, such as CuZnSOD and glutathione reductase, suggest that S. pombe can serve as a good model system to study the effect of oxidative stress on cell proliferation.

Original languageEnglish (US)
Pages (from-to)854-862
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume297
Issue number4
DOIs
StatePublished - 2002

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Vitamin K 3
Schizosaccharomyces
Yeast
Superoxide Dismutase
Cell Cycle
Genes
Cells
Signal transduction
Gene encoding
Oxidative stress
Glutathione Reductase
Thiamine
Cell proliferation
Enzyme activity
Oxidants
Gene expression
Messenger RNA
Cytokinesis
Enzymes
Cell Cycle Checkpoints

Keywords

  • Cell cycle
  • CuZnSOD
  • Fission yeast
  • HO
  • Menadione
  • Oxidative stress
  • Sod1
  • Stationary phase
  • Wis1 pathway

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Regulation and the role of Cu,Zn-containing superoxide dismutase in cell cycle progression of Schizosaccharomyces pombe. / Lee, Joon; Kwon, Eun Soo; Kim, Dong Wook; Cha, Joonseok; Roe, Jung Hye.

In: Biochemical and Biophysical Research Communications, Vol. 297, No. 4, 2002, p. 854-862.

Research output: Contribution to journalArticle

Lee, Joon ; Kwon, Eun Soo ; Kim, Dong Wook ; Cha, Joonseok ; Roe, Jung Hye. / Regulation and the role of Cu,Zn-containing superoxide dismutase in cell cycle progression of Schizosaccharomyces pombe. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 297, No. 4. pp. 854-862.
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