Regulation of ERK1 and ERK2 by glucose and peptide hormones in pancreatic β cells

Don Arnette, Tara Beers Gibson, Michael C. Lawrence, Bridgette January, Shih Khoo, Kathleen McGlynn, Colleen A. Vanderbilt, Melanie H. Cobb

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Abstract

We showed previously that ERK1/2 were activated by glucose and amino acids in pancreatic β cells. Here we examine and compare signaling events that are necessary for ERK1/2 activation by glucose and other stimuli in β cells. We find that agents that interrupt Ca2+ signaling by a variety of mechanisms interfere with glucose- and glucagon-like peptide (GLP-1)-stimulated ERK1/2 activity. In particular, calmodulin antagonists, FK506, and cyclosporin, immunosuppressants that inhibit the calcium-dependent phosphatase calcineurin, suppress ERK1/2 activation by both glucose and GLP-1. Ca2+ signaling from intracellular stores is also essential for ERK1/ 2 activation, because thapsigargin blocks ERK1/2 activation by glucose or GLP-1. The glucosesensitive mechanism is distinct from that used by phorbol ester or insulin to stimulate ERK1/2 but shares common features with that used by GLP-1.

Original languageEnglish (US)
Pages (from-to)32517-32525
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number35
DOIs
StatePublished - Aug 29 2003

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Glucagon-Like Peptide 1
Peptide Hormones
Glucose
Chemical activation
Glucagon-Like Peptides
Thapsigargin
Tacrolimus
Phorbol Esters
Calmodulin
Immunosuppressive Agents
Cyclosporine
Insulin
Calcium
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Arnette, D., Gibson, T. B., Lawrence, M. C., January, B., Khoo, S., McGlynn, K., ... Cobb, M. H. (2003). Regulation of ERK1 and ERK2 by glucose and peptide hormones in pancreatic β cells. Journal of Biological Chemistry, 278(35), 32517-32525. https://doi.org/10.1074/jbc.M301174200

Regulation of ERK1 and ERK2 by glucose and peptide hormones in pancreatic β cells. / Arnette, Don; Gibson, Tara Beers; Lawrence, Michael C.; January, Bridgette; Khoo, Shih; McGlynn, Kathleen; Vanderbilt, Colleen A.; Cobb, Melanie H.

In: Journal of Biological Chemistry, Vol. 278, No. 35, 29.08.2003, p. 32517-32525.

Research output: Contribution to journalArticle

Arnette, D, Gibson, TB, Lawrence, MC, January, B, Khoo, S, McGlynn, K, Vanderbilt, CA & Cobb, MH 2003, 'Regulation of ERK1 and ERK2 by glucose and peptide hormones in pancreatic β cells', Journal of Biological Chemistry, vol. 278, no. 35, pp. 32517-32525. https://doi.org/10.1074/jbc.M301174200
Arnette D, Gibson TB, Lawrence MC, January B, Khoo S, McGlynn K et al. Regulation of ERK1 and ERK2 by glucose and peptide hormones in pancreatic β cells. Journal of Biological Chemistry. 2003 Aug 29;278(35):32517-32525. https://doi.org/10.1074/jbc.M301174200
Arnette, Don ; Gibson, Tara Beers ; Lawrence, Michael C. ; January, Bridgette ; Khoo, Shih ; McGlynn, Kathleen ; Vanderbilt, Colleen A. ; Cobb, Melanie H. / Regulation of ERK1 and ERK2 by glucose and peptide hormones in pancreatic β cells. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 35. pp. 32517-32525.
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