Regulation of gallbladder cholesterol concentration in the hamster. Role of hepatic cholesterol level

John M. Andersen, Laura R. Cook

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The goal of this investigation was to determine how alterations in hepatic cholesterol metabolism influence the cholesterol content of gallbladder bile in hamsters. Although the rate of hepatic cholesterol synthesis was varied over 600-fold, there was no direct relationship between the rate of cholesterol synthesis and the cholesterol content of gallbladder bile. However, expansion of the hepatic cholesterol pool by 42-fold resulted in an 11-fold increase in gallbladder bile cholesterol. Examination of four subfractions of the hepatic cholesterol pool revealed that the cholesterol content of gallbladder bile was most consistently correlated with the free cholesterol level in both hepatic tissue and hepatic microsomes from all experimental groups. In most groups of animals in which gallbladder bile cholesterol was increased, plasma lipoprotein cholesterol levels were also increased. It was concluded that in hamsters, under these experimental conditions, changes in the cholesterol content of gallbladder bile were directly related to alterations in cholesterol content of the liver and most closely related to alterations in the free cholesterol content of that tissue.

Original languageEnglish (US)
Pages (from-to)582-592
Number of pages11
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume875
Issue number3
DOIs
StatePublished - Feb 28 1986

Fingerprint

Gallbladder
Cricetinae
Cholesterol
Liver
Bile
Tissue
Microsomes
Metabolism
Animals

Keywords

  • (Hamster)
  • Bile composition
  • Cholesterol level
  • Cholesterol synthesis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology

Cite this

@article{0d73f1c000584dfc98d3244df554320e,
title = "Regulation of gallbladder cholesterol concentration in the hamster. Role of hepatic cholesterol level",
abstract = "The goal of this investigation was to determine how alterations in hepatic cholesterol metabolism influence the cholesterol content of gallbladder bile in hamsters. Although the rate of hepatic cholesterol synthesis was varied over 600-fold, there was no direct relationship between the rate of cholesterol synthesis and the cholesterol content of gallbladder bile. However, expansion of the hepatic cholesterol pool by 42-fold resulted in an 11-fold increase in gallbladder bile cholesterol. Examination of four subfractions of the hepatic cholesterol pool revealed that the cholesterol content of gallbladder bile was most consistently correlated with the free cholesterol level in both hepatic tissue and hepatic microsomes from all experimental groups. In most groups of animals in which gallbladder bile cholesterol was increased, plasma lipoprotein cholesterol levels were also increased. It was concluded that in hamsters, under these experimental conditions, changes in the cholesterol content of gallbladder bile were directly related to alterations in cholesterol content of the liver and most closely related to alterations in the free cholesterol content of that tissue.",
keywords = "(Hamster), Bile composition, Cholesterol level, Cholesterol synthesis",
author = "Andersen, {John M.} and Cook, {Laura R.}",
year = "1986",
month = "2",
day = "28",
doi = "10.1016/0005-2760(86)90080-9",
language = "English (US)",
volume = "875",
pages = "582--592",
journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",
issn = "1388-1981",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Regulation of gallbladder cholesterol concentration in the hamster. Role of hepatic cholesterol level

AU - Andersen, John M.

AU - Cook, Laura R.

PY - 1986/2/28

Y1 - 1986/2/28

N2 - The goal of this investigation was to determine how alterations in hepatic cholesterol metabolism influence the cholesterol content of gallbladder bile in hamsters. Although the rate of hepatic cholesterol synthesis was varied over 600-fold, there was no direct relationship between the rate of cholesterol synthesis and the cholesterol content of gallbladder bile. However, expansion of the hepatic cholesterol pool by 42-fold resulted in an 11-fold increase in gallbladder bile cholesterol. Examination of four subfractions of the hepatic cholesterol pool revealed that the cholesterol content of gallbladder bile was most consistently correlated with the free cholesterol level in both hepatic tissue and hepatic microsomes from all experimental groups. In most groups of animals in which gallbladder bile cholesterol was increased, plasma lipoprotein cholesterol levels were also increased. It was concluded that in hamsters, under these experimental conditions, changes in the cholesterol content of gallbladder bile were directly related to alterations in cholesterol content of the liver and most closely related to alterations in the free cholesterol content of that tissue.

AB - The goal of this investigation was to determine how alterations in hepatic cholesterol metabolism influence the cholesterol content of gallbladder bile in hamsters. Although the rate of hepatic cholesterol synthesis was varied over 600-fold, there was no direct relationship between the rate of cholesterol synthesis and the cholesterol content of gallbladder bile. However, expansion of the hepatic cholesterol pool by 42-fold resulted in an 11-fold increase in gallbladder bile cholesterol. Examination of four subfractions of the hepatic cholesterol pool revealed that the cholesterol content of gallbladder bile was most consistently correlated with the free cholesterol level in both hepatic tissue and hepatic microsomes from all experimental groups. In most groups of animals in which gallbladder bile cholesterol was increased, plasma lipoprotein cholesterol levels were also increased. It was concluded that in hamsters, under these experimental conditions, changes in the cholesterol content of gallbladder bile were directly related to alterations in cholesterol content of the liver and most closely related to alterations in the free cholesterol content of that tissue.

KW - (Hamster)

KW - Bile composition

KW - Cholesterol level

KW - Cholesterol synthesis

UR - http://www.scopus.com/inward/record.url?scp=0022458522&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022458522&partnerID=8YFLogxK

U2 - 10.1016/0005-2760(86)90080-9

DO - 10.1016/0005-2760(86)90080-9

M3 - Article

C2 - 3947659

AN - SCOPUS:0022458522

VL - 875

SP - 582

EP - 592

JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

SN - 1388-1981

IS - 3

ER -