Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq

Alan V. Smrcka, John R. Hepler, Kendall O. Brown, Paul C. Sternweis

Research output: Contribution to journalArticle

662 Citations (Scopus)

Abstract

The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) by phospholipase C yields the second messengers inositol 1,4,5-trisphosphate (InsP3) and 1,2-diacylglycerol. This activity is regulated by a variety of hormones through G protein pathways. However, the specific G protein or proteins involved has not been identified. The α subunit of a newly discovered pertussis toxin-insensitive G protein (Gq) has recently been isolated and is now shown to stimulate the activity of polyphosphoinositidespecific phospholipase C (PI-PLC) from bovine brain. Both the maximal activity and the affinity of PI-PLC for calcium ion were affected. These results identify Gq as a G protein that regulates PI-PLC.

Original languageEnglish (US)
Pages (from-to)804-807
Number of pages4
JournalScience
Volume251
Issue number4995
StatePublished - Feb 15 1991

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Phosphoinositide Phospholipase C
Type C Phospholipases
GTP-Binding Proteins
Gq-G11 GTP-Binding Protein alpha Subunits
Inositol 1,4,5-Trisphosphate
Pertussis Toxin
Second Messenger Systems
Phosphatidylinositols
Hydrolysis
Hormones
Ions
Calcium
Brain
Proteins

ASJC Scopus subject areas

  • General

Cite this

Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq . / Smrcka, Alan V.; Hepler, John R.; Brown, Kendall O.; Sternweis, Paul C.

In: Science, Vol. 251, No. 4995, 15.02.1991, p. 804-807.

Research output: Contribution to journalArticle

Smrcka, AV, Hepler, JR, Brown, KO & Sternweis, PC 1991, 'Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq ', Science, vol. 251, no. 4995, pp. 804-807.
Smrcka, Alan V. ; Hepler, John R. ; Brown, Kendall O. ; Sternweis, Paul C. / Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq In: Science. 1991 ; Vol. 251, No. 4995. pp. 804-807.
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