Abstract
Type I and type II adenylylcyclases have been purified after expression in Sf9 cells, each by application of a two-step purification protocol. The specific activities of the essentially homogeneous enzymes are approximately 7 and 2 μmol·min-1·mg-1, respectively. Each purified enzyme preparation is activated by Gsα, but they are regulated in an opposite fashion by G protein βγ subunits. Purified βγ inhibits Gsα-stimulated type I adenylylcyclase directly, while βγ activates type II adenylylcyclase and potentiates the Gsα-mediated stimulation of the enzyme. This is the first demonstration of the activation of a purified effector molecule by G protein βγ subunits.
Original language | English (US) |
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Pages (from-to) | 9-12 |
Number of pages | 4 |
Journal | Journal of Biological Chemistry |
Volume | 268 |
Issue number | 1 |
State | Published - Jan 5 1993 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology