Regulation of purified type I and type II adenylylcyclases by G protein βγ subunits

Ronald Taussig, Lynne M. Quarmby, Alfred G. Gilman

Research output: Contribution to journalArticle

192 Scopus citations

Abstract

Type I and type II adenylylcyclases have been purified after expression in Sf9 cells, each by application of a two-step purification protocol. The specific activities of the essentially homogeneous enzymes are approximately 7 and 2 μmol · min-1 · mg-1, respectively. Each purified enzyme preparation is activated by G(sα), but they are regulated in an opposite fashion by G protein βγ subunits. Purified βγ inhibits G(sα)-stimulated type I adenylylcyclase directly, while βγ activates type II adenylylcyclase and potentiates the G(sα)-mediated stimulation of the enzyme. This is the first demonstration of the activation of a purified effector molecule by G protein βγ subunits.

Original languageEnglish (US)
Pages (from-to)9-12
Number of pages4
JournalJournal of Biological Chemistry
Volume268
Issue number1
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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