Regulation of T cell proliferation by anti-CD49d and anti-CD29 monoclonal antibodies

J. L. Bednarczyk, T. K. Teague, J. N. Wygant, L. S. Davis, P. E. Lipsky, B. W. McIntyre

Research output: Contribution to journalArticle

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Abstract

The β1 integrin VLA-4 (α4β1, CD49d/CD29), which is expressed on a large subpopulation of peripheral blood T lymphocytes, functions as a receptor for the endothelial adhesion protein VCAM-1 and the extracellular matrix protein fibronectin. Previous studies showed that immobilized fibronectin enhanced anti-CD3 monoclonal antibody (mAb)-induced T cell proliferation through binding to the integrins VLA-4 and VLA-5 (α5β1, CD49e/CD29). We studied the ability of the anti-CD49d mAb L25 to potentiate proliferation. T cell proliferation was induced by subthreshold concentrations of anti-CD3 mAb (mAb OKT3) coimmobilized with mAb L25 but not with coimmobilized anti-CD29 (β1) mAb. Soluble anti-CD29 mAb inhibited the proliferation induced by coimmobilized mAb OKT3 and L25 but not proliferation induced by mAb OKT3 with PMA or coimmobilized anti-CD26 mAb.

Original languageEnglish (US)
Pages (from-to)456-462
Number of pages7
JournalJournal of Leukocyte Biology
Volume52
Issue number4
StatePublished - 1992

Fingerprint

Integrin alpha4beta1
Monoclonal Antibodies
Cell Proliferation
T-Lymphocytes
Muromonab-CD3
Fibronectins
Integrins
Integrin alpha5beta1
Vascular Cell Adhesion Molecule-1
Extracellular Matrix Proteins

Keywords

  • CD29
  • CD49d
  • integrins
  • VLA-4

ASJC Scopus subject areas

  • Cell Biology

Cite this

Bednarczyk, J. L., Teague, T. K., Wygant, J. N., Davis, L. S., Lipsky, P. E., & McIntyre, B. W. (1992). Regulation of T cell proliferation by anti-CD49d and anti-CD29 monoclonal antibodies. Journal of Leukocyte Biology, 52(4), 456-462.

Regulation of T cell proliferation by anti-CD49d and anti-CD29 monoclonal antibodies. / Bednarczyk, J. L.; Teague, T. K.; Wygant, J. N.; Davis, L. S.; Lipsky, P. E.; McIntyre, B. W.

In: Journal of Leukocyte Biology, Vol. 52, No. 4, 1992, p. 456-462.

Research output: Contribution to journalArticle

Bednarczyk, JL, Teague, TK, Wygant, JN, Davis, LS, Lipsky, PE & McIntyre, BW 1992, 'Regulation of T cell proliferation by anti-CD49d and anti-CD29 monoclonal antibodies', Journal of Leukocyte Biology, vol. 52, no. 4, pp. 456-462.
Bednarczyk JL, Teague TK, Wygant JN, Davis LS, Lipsky PE, McIntyre BW. Regulation of T cell proliferation by anti-CD49d and anti-CD29 monoclonal antibodies. Journal of Leukocyte Biology. 1992;52(4):456-462.
Bednarczyk, J. L. ; Teague, T. K. ; Wygant, J. N. ; Davis, L. S. ; Lipsky, P. E. ; McIntyre, B. W. / Regulation of T cell proliferation by anti-CD49d and anti-CD29 monoclonal antibodies. In: Journal of Leukocyte Biology. 1992 ; Vol. 52, No. 4. pp. 456-462.
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