Regulation of T cell proliferation by anti-CD49d and anti-CD29 monoclonal antibodies

J. L. Bednarczyk, T. K. Teague, J. N. Wygant, L. S. Davis, P. E. Lipsky, B. W. McIntyre

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The β1 integrin VLA-4 (α4β1, CD49d/CD29), which is expressed on a large subpopulation of peripheral blood T lymphocytes, functions as a receptor for the endothelial adhesion protein VCAM-1 and the extracellular matrix protein fibronectin. Previous studies showed that immobilized fibronectin enhanced anti-CD3 monoclonal antibody (mAb)-induced T cell proliferation through binding to the integrins VLA-4 and VLA-5 (α5β1, CD49e/CD29). We studied the ability of the anti-CD49d mAb L25 to potentiate proliferation. T cell proliferation was induced by subthreshold concentrations of anti-CD3 mAb (mAb OKT3) coimmobilized with mAb L25 but not with coimmobilized anti-CD29 (β1) mAb. Soluble anti-CD29 mAb inhibited the proliferation induced by coimmobilized mAb OKT3 and L25 but not proliferation induced by mAb OKT3 with PMA or coimmobilized anti-CD26 mAb.

Original languageEnglish (US)
Pages (from-to)456-462
Number of pages7
JournalJournal of Leukocyte Biology
Volume52
Issue number4
DOIs
StatePublished - Jan 1 1992

Keywords

  • CD29
  • CD49d
  • VLA-4
  • integrins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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    Bednarczyk, J. L., Teague, T. K., Wygant, J. N., Davis, L. S., Lipsky, P. E., & McIntyre, B. W. (1992). Regulation of T cell proliferation by anti-CD49d and anti-CD29 monoclonal antibodies. Journal of Leukocyte Biology, 52(4), 456-462. https://doi.org/10.1002/jlb.52.4.456