Regulation of testosterone secretion by prolactin in male rats

William J. Huang, Yeh Jiun-Yih, Shiow Chwen Tsai, Ho Lin, Yu Chung Chiao, Jiann Jong Chen, Chien Chen Lu, Seng Wong Hwang, Shyi Wu Wang, Luke S. Chang, Paulus S. Wang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The goal of this study was to characterize the mechanism by which hyperprolactinemia alters testosterone production in rat testicular interstitial cells (TICs). Hyperprolactinemia was induced by grafting 2 anterior pituitary (AP) glands under the subcapsular space of the kidney in experimental rats. Control rats were grafted with brain cortex (CX). Six weeks post-grafting, rats were challenged with human chorionic gonadotropin (hCG) then, the changes in either plasma testosterone or luteinizing hormone was measured. Additionally, TICs were isolated and challenged in vitro with hCG or prolactin, and the testosterone release measured by radioimmunoassay. Further investigation in signal transduction as intracellular 3':5' cyclic adenosine monophosphate (cAMP) production was observed under a regulation of forskolin or SQ22536. After the challenge of hCG or GnRH, the AP-grafted rats showed a suppressed response in testosterone release as compared to those in the CX-grafted group. The in vitro data from the AP-grafted rats compared to the CX-grafted animals showed a diminished response in testosterone release upon hCG stimulation. Administration of forskolin or SQ22536 disclosed dysfunction of adenylate cyclase in TICs from the AP-grafted rats. When 8- Br-cAMP was incubated with TICs, the testosterone production was lower in the AP-grafted compared to the CX-grafted group. These results suggest that in addition to adenylate cyclase dysfunction, inefficiency of post-cAMP pathways are also involved in the hypogonadism elicited by hyperprolactinemia in rats.

Original languageEnglish (US)
Pages (from-to)111-118
Number of pages8
JournalJournal of Cellular Biochemistry
Volume74
Issue number1
DOIs
StatePublished - Jul 1 1999

Fingerprint

Prolactin
Testosterone
Rats
Chorionic Gonadotropin
Leydig Cells
Hyperprolactinemia
Cyclic AMP
Colforsin
Adenylyl Cyclases
Rat control
Signal transduction
Anterior Pituitary Gland
Hypogonadism
Luteinizing Hormone
Gonadotropin-Releasing Hormone
Brain
Animals
Radioimmunoassay
Signal Transduction
Plasmas

Keywords

  • CAMP
  • Hyperprolactinemia
  • Male rats
  • PRL
  • Testosterone

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Huang, W. J., Jiun-Yih, Y., Tsai, S. C., Lin, H., Chiao, Y. C., Chen, J. J., ... Wang, P. S. (1999). Regulation of testosterone secretion by prolactin in male rats. Journal of Cellular Biochemistry, 74(1), 111-118. https://doi.org/10.1002/(SICI)1097-4644(19990701)74:1<111::AID-JCB12>3.0.CO;2-I

Regulation of testosterone secretion by prolactin in male rats. / Huang, William J.; Jiun-Yih, Yeh; Tsai, Shiow Chwen; Lin, Ho; Chiao, Yu Chung; Chen, Jiann Jong; Lu, Chien Chen; Hwang, Seng Wong; Wang, Shyi Wu; Chang, Luke S.; Wang, Paulus S.

In: Journal of Cellular Biochemistry, Vol. 74, No. 1, 01.07.1999, p. 111-118.

Research output: Contribution to journalArticle

Huang, WJ, Jiun-Yih, Y, Tsai, SC, Lin, H, Chiao, YC, Chen, JJ, Lu, CC, Hwang, SW, Wang, SW, Chang, LS & Wang, PS 1999, 'Regulation of testosterone secretion by prolactin in male rats', Journal of Cellular Biochemistry, vol. 74, no. 1, pp. 111-118. https://doi.org/10.1002/(SICI)1097-4644(19990701)74:1<111::AID-JCB12>3.0.CO;2-I
Huang, William J. ; Jiun-Yih, Yeh ; Tsai, Shiow Chwen ; Lin, Ho ; Chiao, Yu Chung ; Chen, Jiann Jong ; Lu, Chien Chen ; Hwang, Seng Wong ; Wang, Shyi Wu ; Chang, Luke S. ; Wang, Paulus S. / Regulation of testosterone secretion by prolactin in male rats. In: Journal of Cellular Biochemistry. 1999 ; Vol. 74, No. 1. pp. 111-118.
@article{a71d00b3ff5443199c9da08936a2ada5,
title = "Regulation of testosterone secretion by prolactin in male rats",
abstract = "The goal of this study was to characterize the mechanism by which hyperprolactinemia alters testosterone production in rat testicular interstitial cells (TICs). Hyperprolactinemia was induced by grafting 2 anterior pituitary (AP) glands under the subcapsular space of the kidney in experimental rats. Control rats were grafted with brain cortex (CX). Six weeks post-grafting, rats were challenged with human chorionic gonadotropin (hCG) then, the changes in either plasma testosterone or luteinizing hormone was measured. Additionally, TICs were isolated and challenged in vitro with hCG or prolactin, and the testosterone release measured by radioimmunoassay. Further investigation in signal transduction as intracellular 3':5' cyclic adenosine monophosphate (cAMP) production was observed under a regulation of forskolin or SQ22536. After the challenge of hCG or GnRH, the AP-grafted rats showed a suppressed response in testosterone release as compared to those in the CX-grafted group. The in vitro data from the AP-grafted rats compared to the CX-grafted animals showed a diminished response in testosterone release upon hCG stimulation. Administration of forskolin or SQ22536 disclosed dysfunction of adenylate cyclase in TICs from the AP-grafted rats. When 8- Br-cAMP was incubated with TICs, the testosterone production was lower in the AP-grafted compared to the CX-grafted group. These results suggest that in addition to adenylate cyclase dysfunction, inefficiency of post-cAMP pathways are also involved in the hypogonadism elicited by hyperprolactinemia in rats.",
keywords = "CAMP, Hyperprolactinemia, Male rats, PRL, Testosterone",
author = "Huang, {William J.} and Yeh Jiun-Yih and Tsai, {Shiow Chwen} and Ho Lin and Chiao, {Yu Chung} and Chen, {Jiann Jong} and Lu, {Chien Chen} and Hwang, {Seng Wong} and Wang, {Shyi Wu} and Chang, {Luke S.} and Wang, {Paulus S.}",
year = "1999",
month = "7",
day = "1",
doi = "10.1002/(SICI)1097-4644(19990701)74:1<111::AID-JCB12>3.0.CO;2-I",
language = "English (US)",
volume = "74",
pages = "111--118",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Regulation of testosterone secretion by prolactin in male rats

AU - Huang, William J.

AU - Jiun-Yih, Yeh

AU - Tsai, Shiow Chwen

AU - Lin, Ho

AU - Chiao, Yu Chung

AU - Chen, Jiann Jong

AU - Lu, Chien Chen

AU - Hwang, Seng Wong

AU - Wang, Shyi Wu

AU - Chang, Luke S.

AU - Wang, Paulus S.

PY - 1999/7/1

Y1 - 1999/7/1

N2 - The goal of this study was to characterize the mechanism by which hyperprolactinemia alters testosterone production in rat testicular interstitial cells (TICs). Hyperprolactinemia was induced by grafting 2 anterior pituitary (AP) glands under the subcapsular space of the kidney in experimental rats. Control rats were grafted with brain cortex (CX). Six weeks post-grafting, rats were challenged with human chorionic gonadotropin (hCG) then, the changes in either plasma testosterone or luteinizing hormone was measured. Additionally, TICs were isolated and challenged in vitro with hCG or prolactin, and the testosterone release measured by radioimmunoassay. Further investigation in signal transduction as intracellular 3':5' cyclic adenosine monophosphate (cAMP) production was observed under a regulation of forskolin or SQ22536. After the challenge of hCG or GnRH, the AP-grafted rats showed a suppressed response in testosterone release as compared to those in the CX-grafted group. The in vitro data from the AP-grafted rats compared to the CX-grafted animals showed a diminished response in testosterone release upon hCG stimulation. Administration of forskolin or SQ22536 disclosed dysfunction of adenylate cyclase in TICs from the AP-grafted rats. When 8- Br-cAMP was incubated with TICs, the testosterone production was lower in the AP-grafted compared to the CX-grafted group. These results suggest that in addition to adenylate cyclase dysfunction, inefficiency of post-cAMP pathways are also involved in the hypogonadism elicited by hyperprolactinemia in rats.

AB - The goal of this study was to characterize the mechanism by which hyperprolactinemia alters testosterone production in rat testicular interstitial cells (TICs). Hyperprolactinemia was induced by grafting 2 anterior pituitary (AP) glands under the subcapsular space of the kidney in experimental rats. Control rats were grafted with brain cortex (CX). Six weeks post-grafting, rats were challenged with human chorionic gonadotropin (hCG) then, the changes in either plasma testosterone or luteinizing hormone was measured. Additionally, TICs were isolated and challenged in vitro with hCG or prolactin, and the testosterone release measured by radioimmunoassay. Further investigation in signal transduction as intracellular 3':5' cyclic adenosine monophosphate (cAMP) production was observed under a regulation of forskolin or SQ22536. After the challenge of hCG or GnRH, the AP-grafted rats showed a suppressed response in testosterone release as compared to those in the CX-grafted group. The in vitro data from the AP-grafted rats compared to the CX-grafted animals showed a diminished response in testosterone release upon hCG stimulation. Administration of forskolin or SQ22536 disclosed dysfunction of adenylate cyclase in TICs from the AP-grafted rats. When 8- Br-cAMP was incubated with TICs, the testosterone production was lower in the AP-grafted compared to the CX-grafted group. These results suggest that in addition to adenylate cyclase dysfunction, inefficiency of post-cAMP pathways are also involved in the hypogonadism elicited by hyperprolactinemia in rats.

KW - CAMP

KW - Hyperprolactinemia

KW - Male rats

KW - PRL

KW - Testosterone

UR - http://www.scopus.com/inward/record.url?scp=0345465668&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345465668&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-4644(19990701)74:1<111::AID-JCB12>3.0.CO;2-I

DO - 10.1002/(SICI)1097-4644(19990701)74:1<111::AID-JCB12>3.0.CO;2-I

M3 - Article

C2 - 10381267

AN - SCOPUS:0345465668

VL - 74

SP - 111

EP - 118

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 1

ER -