Relationship between gangliosides and doubling times in cultured human brain and brain tumor cells

Mary Thoesen Coleman; Lawrence E. Allred; Ronald W. Hart; Allan J. Yates

doi:10.1016/0304-3835(80)90011-7

Relationship between gangliosides and doubling times in cultured human brain and brain tumor cells

Mary Thoesen Coleman, Lawrence E. Allred, Ronald W. Hart, Allan J. Yates

Health Care Sciences

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Abstract

Cells cultured in vitro from normal human white matter and 3 individual brain tumors exhibited the following population doubling times: cells from normal human white matter, 64 ± 22 h, glioblastoma multiforme, 43 ± 11 h; malignant astrocytoma, 98 ± 19 h; and anaplastic oligodendroglioma, 81 ± 24 h. Cells were seeded at subconfluent density, pulse-labelled for 24 h with the ganglioside precursor D-[1-¹⁴C]glucosamine and harvested. Radioactive patterns of extracted gangliosides showed that incorporation of label into disialoganglioside was significantly higher in the samples with longer population doubling times than in samples with shorter population doubling times. These findings suggest gangliosides may play a role in regulation of cell growth.

Original language	English (US)
Pages (from-to)	255-262
Number of pages	8
Journal	Cancer Letters
Volume	8
Issue number	3
DOIs	https://doi.org/10.1016/0304-3835(80)90011-7
State	Published - Jan 1980

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/0304-3835(80)90011-7

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@article{55577c8d60e1488ab387f32ff5ba3388,

title = "Relationship between gangliosides and doubling times in cultured human brain and brain tumor cells",

abstract = "Cells cultured in vitro from normal human white matter and 3 individual brain tumors exhibited the following population doubling times: cells from normal human white matter, 64 ± 22 h, glioblastoma multiforme, 43 ± 11 h; malignant astrocytoma, 98 ± 19 h; and anaplastic oligodendroglioma, 81 ± 24 h. Cells were seeded at subconfluent density, pulse-labelled for 24 h with the ganglioside precursor D-[1-14C]glucosamine and harvested. Radioactive patterns of extracted gangliosides showed that incorporation of label into disialoganglioside was significantly higher in the samples with longer population doubling times than in samples with shorter population doubling times. These findings suggest gangliosides may play a role in regulation of cell growth.",

author = "Coleman, {Mary Thoesen} and Allred, {Lawrence E.} and Hart, {Ronald W.} and Yates, {Allan J.}",

note = "Funding Information: This work was supported by The American Cancer Society, Central Ohio Division, and Association for Brain Tumor Research. M.T. Coleman received support as a postdoctoral trainee from the National Institute of Health, Grant No. 5 T 32 N50 7091-02.",

year = "1980",

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TY - JOUR

T1 - Relationship between gangliosides and doubling times in cultured human brain and brain tumor cells

AU - Coleman, Mary Thoesen

AU - Allred, Lawrence E.

AU - Hart, Ronald W.

AU - Yates, Allan J.

N1 - Funding Information: This work was supported by The American Cancer Society, Central Ohio Division, and Association for Brain Tumor Research. M.T. Coleman received support as a postdoctoral trainee from the National Institute of Health, Grant No. 5 T 32 N50 7091-02.

PY - 1980/1

Y1 - 1980/1

N2 - Cells cultured in vitro from normal human white matter and 3 individual brain tumors exhibited the following population doubling times: cells from normal human white matter, 64 ± 22 h, glioblastoma multiforme, 43 ± 11 h; malignant astrocytoma, 98 ± 19 h; and anaplastic oligodendroglioma, 81 ± 24 h. Cells were seeded at subconfluent density, pulse-labelled for 24 h with the ganglioside precursor D-[1-14C]glucosamine and harvested. Radioactive patterns of extracted gangliosides showed that incorporation of label into disialoganglioside was significantly higher in the samples with longer population doubling times than in samples with shorter population doubling times. These findings suggest gangliosides may play a role in regulation of cell growth.

AB - Cells cultured in vitro from normal human white matter and 3 individual brain tumors exhibited the following population doubling times: cells from normal human white matter, 64 ± 22 h, glioblastoma multiforme, 43 ± 11 h; malignant astrocytoma, 98 ± 19 h; and anaplastic oligodendroglioma, 81 ± 24 h. Cells were seeded at subconfluent density, pulse-labelled for 24 h with the ganglioside precursor D-[1-14C]glucosamine and harvested. Radioactive patterns of extracted gangliosides showed that incorporation of label into disialoganglioside was significantly higher in the samples with longer population doubling times than in samples with shorter population doubling times. These findings suggest gangliosides may play a role in regulation of cell growth.

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Relationship between gangliosides and doubling times in cultured human brain and brain tumor cells

Abstract

ASJC Scopus subject areas

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