Replica plating of mammalian cells using low melt agarose

Shobhana Natarajan, Tracy T. Chow, Jerry W. Shay, Woodring E. Wright

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have developed a technique to replica plate mammalian cells grown on plastic dishes using low melt agarose. This method is simpler than previously described methods that use polyester membranes to grow and transfer cells. We have tested the effectiveness of this technique on normal and immortal cell lines and have found that we can transfer cells with an efficiency of 80-90%. We have used this technique to rapidly screen clones for insertion of a lentivirally encoded gene without a selectable marker.

Original languageEnglish (US)
Pages (from-to)145-147
Number of pages3
JournalCytotechnology
Volume54
Issue number3
DOIs
StatePublished - Jul 2007

Fingerprint

Plating
Sepharose
Cells
Polyesters
Replica Techniques
Genes
Plastics
Membranes
Clone Cells
Cell Line

Keywords

  • Cell culture
  • Replica plating
  • Ring cloning
  • Screening
  • Transfection

ASJC Scopus subject areas

  • Cell Biology
  • Biotechnology
  • Clinical Biochemistry

Cite this

Replica plating of mammalian cells using low melt agarose. / Natarajan, Shobhana; Chow, Tracy T.; Shay, Jerry W.; Wright, Woodring E.

In: Cytotechnology, Vol. 54, No. 3, 07.2007, p. 145-147.

Research output: Contribution to journalArticle

Natarajan, Shobhana ; Chow, Tracy T. ; Shay, Jerry W. ; Wright, Woodring E. / Replica plating of mammalian cells using low melt agarose. In: Cytotechnology. 2007 ; Vol. 54, No. 3. pp. 145-147.
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