Replica plating of mammalian cells using low melt agarose

Shobhana Natarajan, Tracy T. Chow, Jerry W. Shay, Woodring E. Wright

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We have developed a technique to replica plate mammalian cells grown on plastic dishes using low melt agarose. This method is simpler than previously described methods that use polyester membranes to grow and transfer cells. We have tested the effectiveness of this technique on normal and immortal cell lines and have found that we can transfer cells with an efficiency of 80-90%. We have used this technique to rapidly screen clones for insertion of a lentivirally encoded gene without a selectable marker.

Original languageEnglish (US)
Pages (from-to)145-147
Number of pages3
JournalCytotechnology
Volume54
Issue number3
DOIs
StatePublished - Jul 1 2007

Keywords

  • Cell culture
  • Replica plating
  • Ring cloning
  • Screening
  • Transfection

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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