Repression of transcription of the p27Kip1 cyclin-dependent kinase inhibitor gene by c-Myc

William Yang, Jian Shen, Min Wu, Marcello Arsura, Mark FitzGerald, Zalman Suldan, Dong W. Kim, Claudia S. Hofmann, Stefania Pianetti, Raphaëlle Romieu-Mourez, Leonard P. Freedman, Gail E. Sonenshein

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

Upon engagement of the B Cell Receptor (BCR) of WEHI 231 immature B cells, a drop in c-Myc expression is followed by activation of the cyclin-dependent kinase inhibitor (CKI) p27Kip1, which induces growth arrest and apoptosis. Here, we report inverse patterns of p27 and c-Myc protein expression follow BCR engagement. We present evidence demonstrating, for the first time, that the p27Kip1 gene is a target of transcriptional repression by c-Myc. Specifically, the changes in p27 protein levels correlated with changes in p27 mRNA levels, and gene transcription. Induction of p27 promoter activity followed BCR engagement of WEHI 231 cells, and this induction could be repressed upon co-transfection of a c-Myc expression vector. Inhibition of the TATA-less p27 promoter by c-Myc was also observed in Jurkat T cells, vascular smooth muscle, and Hs578T breast cancer cells, extending the observation beyond immune cells. Consistent with a putative Inr element CCAGACC (where + 1 is underlined) at the start site of transcription in the p27 promoter, deletion of Myc homology box II reduced the extent of repression. Furthermore, enhanced repression was observed upon transfection of the c-Myc 'superrepressor', with mutation of Phe115 to Leu. The sequences mediating transcriptional activity and c-Myc repression were mapped to bp -20 to + 20 of the p27 gene. Finally, binding of Max was shown to facilitate e-Myc binding and repression of p27 promoter activity. Overall, these studies identify the p27 CKl gene as a new target whereby c-Myc can control cell proliferation, survival and neoplastic transformation.

Original languageEnglish (US)
Pages (from-to)1688-1702
Number of pages15
JournalOncogene
Volume20
Issue number14
DOIs
StatePublished - Mar 29 2001

Fingerprint

myc Genes
Cyclin-Dependent Kinases
B-Lymphocytes
Genes
Transfection
Proto-Oncogene Proteins c-myc
Jurkat Cells
B-Lymphoid Precursor Cells
Transcription Initiation Site
Vascular Smooth Muscle
Cell Survival
Cell Proliferation
Observation
Apoptosis
Breast Neoplasms
T-Lymphocytes
Messenger RNA
Mutation
Growth
Proteins

Keywords

  • Breast cancer
  • c-Myc
  • Max
  • p27
  • Smooth muscle cells
  • WEHI 231 cells

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Yang, W., Shen, J., Wu, M., Arsura, M., FitzGerald, M., Suldan, Z., ... Sonenshein, G. E. (2001). Repression of transcription of the p27Kip1 cyclin-dependent kinase inhibitor gene by c-Myc. Oncogene, 20(14), 1688-1702. https://doi.org/10.1038/sj.onc.1204245

Repression of transcription of the p27Kip1 cyclin-dependent kinase inhibitor gene by c-Myc. / Yang, William; Shen, Jian; Wu, Min; Arsura, Marcello; FitzGerald, Mark; Suldan, Zalman; Kim, Dong W.; Hofmann, Claudia S.; Pianetti, Stefania; Romieu-Mourez, Raphaëlle; Freedman, Leonard P.; Sonenshein, Gail E.

In: Oncogene, Vol. 20, No. 14, 29.03.2001, p. 1688-1702.

Research output: Contribution to journalArticle

Yang, W, Shen, J, Wu, M, Arsura, M, FitzGerald, M, Suldan, Z, Kim, DW, Hofmann, CS, Pianetti, S, Romieu-Mourez, R, Freedman, LP & Sonenshein, GE 2001, 'Repression of transcription of the p27Kip1 cyclin-dependent kinase inhibitor gene by c-Myc', Oncogene, vol. 20, no. 14, pp. 1688-1702. https://doi.org/10.1038/sj.onc.1204245
Yang W, Shen J, Wu M, Arsura M, FitzGerald M, Suldan Z et al. Repression of transcription of the p27Kip1 cyclin-dependent kinase inhibitor gene by c-Myc. Oncogene. 2001 Mar 29;20(14):1688-1702. https://doi.org/10.1038/sj.onc.1204245
Yang, William ; Shen, Jian ; Wu, Min ; Arsura, Marcello ; FitzGerald, Mark ; Suldan, Zalman ; Kim, Dong W. ; Hofmann, Claudia S. ; Pianetti, Stefania ; Romieu-Mourez, Raphaëlle ; Freedman, Leonard P. ; Sonenshein, Gail E. / Repression of transcription of the p27Kip1 cyclin-dependent kinase inhibitor gene by c-Myc. In: Oncogene. 2001 ; Vol. 20, No. 14. pp. 1688-1702.
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AU - Suldan, Zalman

AU - Kim, Dong W.

AU - Hofmann, Claudia S.

AU - Pianetti, Stefania

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