Respiratory distress after intratracheal bleomycin: Selective deficiency of surfactant proteins B and C

Rashmin C. Savani, Rodolfo I. Godinez, Marye H. Godinez, Erica Wentz, Aisha Zaman, Cui Zheng, Patricia M. Pooler, Susan H. Guttentag, Michael F. Beers, Linda W. Gonzales, Philip L. Ballard

Research output: Contribution to journalArticle

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Abstract

Intratracheal bleomycin in rats is associated with respiratory distress of uncertain etiology. We investigated the expression of surfactant components in this model of lung injury. Maximum respiratory distress, determined by respiratory rate, occurred at 7 days, and surfactant dysfunction was confirmed by increased surface tension of the large-aggregate fraction of bronchoalveolar lavage (BAL). In injured animals, phospholipid content and composition were similar to those of controls, mature surfactant protein (SP) B was decreased 90%, and SP-A and SP-D contents were increased. In lung tissue, SP-B and SP-C mRNAs were decreased by 2 days and maximally at 4-7 days and recovered between 14 and 21 days after injury. Immunostaining of SP-B and proSP-C was decreased in type II epithelial cells but strong in macrophages. By electron microscopy, injured lungs had type II cells lacking lamellar bodies and macrophages with phagocytosed lamellar bodies. Surface activity of BAL phospholipids of injured animals was restored by addition of exogenous SP-B. We conclude that respiratory distress after bleomycin in rats results from surfactant dysfunction in part secondary to selective downregulation of SP-B and SP-C.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume281
Issue number3 25-3
StatePublished - 2001

Fingerprint

Bleomycin
Protein C
Surface-Active Agents
Bronchoalveolar Lavage
Phospholipids
Macrophages
Pulmonary Surfactant-Associated Protein D
Pulmonary Surfactant-Associated Protein A
Lung
Surface Tension
Lung Injury
Respiratory Rate
Phagocytosis
Electron Microscopy
Down-Regulation
Epithelial Cells
IgA receptor
Messenger RNA
Wounds and Injuries

Keywords

  • Inflammation
  • Lung injury
  • Surface tension
  • Surfactant hydrophobic proteins

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Respiratory distress after intratracheal bleomycin : Selective deficiency of surfactant proteins B and C. / Savani, Rashmin C.; Godinez, Rodolfo I.; Godinez, Marye H.; Wentz, Erica; Zaman, Aisha; Zheng, Cui; Pooler, Patricia M.; Guttentag, Susan H.; Beers, Michael F.; Gonzales, Linda W.; Ballard, Philip L.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 281, No. 3 25-3, 2001.

Research output: Contribution to journalArticle

Savani, RC, Godinez, RI, Godinez, MH, Wentz, E, Zaman, A, Zheng, C, Pooler, PM, Guttentag, SH, Beers, MF, Gonzales, LW & Ballard, PL 2001, 'Respiratory distress after intratracheal bleomycin: Selective deficiency of surfactant proteins B and C', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 281, no. 3 25-3.
Savani, Rashmin C. ; Godinez, Rodolfo I. ; Godinez, Marye H. ; Wentz, Erica ; Zaman, Aisha ; Zheng, Cui ; Pooler, Patricia M. ; Guttentag, Susan H. ; Beers, Michael F. ; Gonzales, Linda W. ; Ballard, Philip L. / Respiratory distress after intratracheal bleomycin : Selective deficiency of surfactant proteins B and C. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2001 ; Vol. 281, No. 3 25-3.
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