Response of X-ray-sensitive CHO mutant cells (xrs-6c) to radiation. II. Relationship between cell survival and the induction of chromosomal damage with low doses of α particles

H. Nagasawa, J. B. Little, W. C. Inkret, S. Carpenter, M. R. Raju, D. J. Chen, G. F. Strniste

Research output: Contribution to journalArticle

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Abstract

The induction of cytotoxicity, chromosomal aberrations, and sister chromatid exchanges (SCEs) was measured in CHO K-1c cells and in isogenic X-ray-sensitive mutant xrs-6c cells that had been irradiated with X rays and α particles in isoleucine-deficient α-minimal essential medium in G1 phase of the cell cycle. There was a noticeable shoulder region on the survival curve for CHO K-1c cells irradiated with very low doses of α particles, whereas this feature was absent for xrs-6c cells with α-particle doses as low as 0.5 cGy. Higher frequencies of chromatid-type aberrations were induced in G1-phase xrs-6c cells than in G1-phase CHO K-1c cells by both γ- and α-particle irradiation. Induction of nonlethal chromosomal aberrations was observed following exposure to 2-6 cGy of α particles, doses yielding 97-100% cell survival. Irradiation with 0.5 cGy of α particles induced SCE; nearly 60% of irradiated cells contained significantly increased levels of SCE. However, only 3% of the nuclei of cells exposed to 0.5 cGy of α-particle radiation were actually traversed by an α particle. The observation that a large fraction of cells apparently survive exposure to very low doses of α-particle radiation with persistent genetic damage manifested by both chromosomal aberrations and SCEs may have important implications for the carcinogenic hazards of high-LET radiation.

Original languageEnglish (US)
Pages (from-to)280-288
Number of pages9
JournalRadiation Research
Volume126
Issue number3
StatePublished - 1991

Fingerprint

CHO Cells
cell viability
Cell Survival
induction
X-radiation
X-Rays
Radiation
damage
dosage
mutants
Sister Chromatid Exchange
radiation
crossing over
cells
G1 Phase
x rays
chromosome aberrations
aberration
Chromosome Aberrations
interphase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Nagasawa, H., Little, J. B., Inkret, W. C., Carpenter, S., Raju, M. R., Chen, D. J., & Strniste, G. F. (1991). Response of X-ray-sensitive CHO mutant cells (xrs-6c) to radiation. II. Relationship between cell survival and the induction of chromosomal damage with low doses of α particles. Radiation Research, 126(3), 280-288.

Response of X-ray-sensitive CHO mutant cells (xrs-6c) to radiation. II. Relationship between cell survival and the induction of chromosomal damage with low doses of α particles. / Nagasawa, H.; Little, J. B.; Inkret, W. C.; Carpenter, S.; Raju, M. R.; Chen, D. J.; Strniste, G. F.

In: Radiation Research, Vol. 126, No. 3, 1991, p. 280-288.

Research output: Contribution to journalArticle

Nagasawa, H. ; Little, J. B. ; Inkret, W. C. ; Carpenter, S. ; Raju, M. R. ; Chen, D. J. ; Strniste, G. F. / Response of X-ray-sensitive CHO mutant cells (xrs-6c) to radiation. II. Relationship between cell survival and the induction of chromosomal damage with low doses of α particles. In: Radiation Research. 1991 ; Vol. 126, No. 3. pp. 280-288.
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abstract = "The induction of cytotoxicity, chromosomal aberrations, and sister chromatid exchanges (SCEs) was measured in CHO K-1c cells and in isogenic X-ray-sensitive mutant xrs-6c cells that had been irradiated with X rays and α particles in isoleucine-deficient α-minimal essential medium in G1 phase of the cell cycle. There was a noticeable shoulder region on the survival curve for CHO K-1c cells irradiated with very low doses of α particles, whereas this feature was absent for xrs-6c cells with α-particle doses as low as 0.5 cGy. Higher frequencies of chromatid-type aberrations were induced in G1-phase xrs-6c cells than in G1-phase CHO K-1c cells by both γ- and α-particle irradiation. Induction of nonlethal chromosomal aberrations was observed following exposure to 2-6 cGy of α particles, doses yielding 97-100{\%} cell survival. Irradiation with 0.5 cGy of α particles induced SCE; nearly 60{\%} of irradiated cells contained significantly increased levels of SCE. However, only 3{\%} of the nuclei of cells exposed to 0.5 cGy of α-particle radiation were actually traversed by an α particle. The observation that a large fraction of cells apparently survive exposure to very low doses of α-particle radiation with persistent genetic damage manifested by both chromosomal aberrations and SCEs may have important implications for the carcinogenic hazards of high-LET radiation.",
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