Reversible Dissociation of Wheat Germ Ribosomal Subunits: Cation-Dependent Equilibriums and Thermodynamic Parameters

Joan M. Sperrazza, David W. Russell, Linda L. Spremulli

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The influence of magnesium ion concentration upon the equilibrium between the wheat germ ribosome and its subunits has been studied by light scattering. The curves obtained for ribosome dissociation and subunit reassociation were identical and were independent of the origin and direction of the magnesium ion titration, suggesting that the wheat germ ribosomes are behaving as a homogeneous population equivalent to Escherichia coli type A ribosomes. Increasing K+ concentrations in the presence of Mg2+ favored ribosome dissociation. Polyamines favored subunit reassociation, with 0.1 mM spermine acting as effectively as 1 mM spermidine.

Original languageEnglish (US)
Pages (from-to)1053-1058
Number of pages6
JournalBiochemistry
Volume19
Issue number6
DOIs
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Biochemistry

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