Rhythmic expression of Nocturnin mRNA in multiple tissues of the mouse

Yunxia Wang, David L. Osterbur, Pamela L. Megaw, Gianluca Tosini, Chiaki Fukuhara, Carla B. Green, Joseph C. Besharse

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Background: Nocturnin was originally identified by differential display as a circadian clock regulated gene with high expression at night in photoreceptors of the African clawed frog, Xenopus laevis. Although encoding a novel protein, the nocturnin cDNA had strong sequence similarity with a C-terminal domain of the yeast transcription factor CCR4, and with mouse and human ESTs. Since its original identification others have cloned mouse and human homologues of nocturnin/CCR4, and we have cloned a full-length cDNA from mouse retina, along with partial cDNAs from human, cow and chicken. The goal of this study was to determine the temporal pattern of nocturnin mRNA expression in multiple tissues of the mouse. Results: cDNA sequence analysis revealed a high degree of conservation among vertebrate nocturnin/CCR4 homologues along with a possible homologue in Drosophila. Northern analysis of mRNA in C3H/He and C57/B16 mice revealed that the mNoc gene is expressed in a broad range of tissues, with greatest abundance in liver, kidney and testis. mNoc is also expressed in multiple brain regions including suprachiasmatic nucleus and pineal gland. Furthermore, mNoc exhibits circadian rhythmicity of mRNA abundance with peak levels at the time of light offset in the retina, spleen, heart, kidney and liver. Conclusion: The widespread expression and rhythmicity of mNoc mRNA parallels the widespread expression of other circadian clock genes in mammalian tissues, and suggests that nocturnin plays an important role in clock function or as a circadian clock effector.

Original languageEnglish (US)
Article number1
Pages (from-to)1-15
Number of pages15
JournalBMC Developmental Biology
Volume1
DOIs
StatePublished - May 25 2001

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Messenger RNA
Circadian Clocks
Complementary DNA
Periodicity
Retina
Genes
Kidney
Suprachiasmatic Nucleus
Pineal Gland
Liver
Expressed Sequence Tags
Xenopus laevis
Anura
Drosophila
Sequence Analysis
Vertebrates
nocturnin
Testis
Chickens
Transcription Factors

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Wang, Y., Osterbur, D. L., Megaw, P. L., Tosini, G., Fukuhara, C., Green, C. B., & Besharse, J. C. (2001). Rhythmic expression of Nocturnin mRNA in multiple tissues of the mouse. BMC Developmental Biology, 1, 1-15. [1]. https://doi.org/10.1186/1471-213X-1-1

Rhythmic expression of Nocturnin mRNA in multiple tissues of the mouse. / Wang, Yunxia; Osterbur, David L.; Megaw, Pamela L.; Tosini, Gianluca; Fukuhara, Chiaki; Green, Carla B.; Besharse, Joseph C.

In: BMC Developmental Biology, Vol. 1, 1, 25.05.2001, p. 1-15.

Research output: Contribution to journalArticle

Wang, Y, Osterbur, DL, Megaw, PL, Tosini, G, Fukuhara, C, Green, CB & Besharse, JC 2001, 'Rhythmic expression of Nocturnin mRNA in multiple tissues of the mouse', BMC Developmental Biology, vol. 1, 1, pp. 1-15. https://doi.org/10.1186/1471-213X-1-1
Wang, Yunxia ; Osterbur, David L. ; Megaw, Pamela L. ; Tosini, Gianluca ; Fukuhara, Chiaki ; Green, Carla B. ; Besharse, Joseph C. / Rhythmic expression of Nocturnin mRNA in multiple tissues of the mouse. In: BMC Developmental Biology. 2001 ; Vol. 1. pp. 1-15.
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