@article{72d8b45f93e2461e9bc6bd861ff7b204,
title = "Rna promotes phase separation of glycolysis enzymes into yeast g bodies in hypoxia",
abstract = "In hypoxic stress conditions, glycolysis enzymes assemble into singular cytoplasmic granules called glycolytic (G) bodies. G body formation in yeast correlates with increased glucose consumption and cell survival. However, the physical properties and organizing principles that define G body formation are unclear. We demonstrate that glycolysis enzymes are non-canonical RNA binding proteins, sharing many common mRNA substrates that are also integral constituents of G bodies. Targeting nonspecific endoribonucleases to G bodies reveals that RNA nucleates G body formation and maintains its structural integrity. Consistent with a phase separation mechanism of biogenesis, recruitment of glycolysis enzymes to G bodies relies on multivalent homotypic and heterotypic interactions. Furthermore, G bodies fuse in vivo and are largely insensitive to 1,6-hexanediol, consistent with a hydrogel-like composition. Taken together, our results elucidate the biophysical nature of G bodies and demonstrate that RNA nucleates phase separation of the glycolysis machinery in response to hypoxic stress.",
author = "Fuller, {Gregory G.} and Ting Han and Freeberg, {Mallory A.} and Moresco, {James J.} and Niaki, {Amirhossein Ghanbari} and Roach, {Nathan P.} and Yates, {John R.} and Sua Myong and Kim, {John K.}",
note = "Funding Information: We thank members of the Kim Lab (Amelia Alessi, Charlotte Choi, Mindy Clark, Jessica Kirshner, Alex Rittenhouse, Margaret Starostik, and Rebecca Tay) and Tatjana Trcek for helpful suggestions. We also thank Himani Galagali and Angela Anderson for comments on the manuscript. This work was supported by a grant from the NIH (R01GM129301) and a grant from the National Institute of Neurological Disorders and Stroke, National Alzheimer?s Project Act (NAPA) 1-RF1-NS113636-01. Funding Information: We thank members of the Kim Lab (Amelia Alessi, Charlotte Choi, Mindy Clark, Jessica Kirshner, Alex Rittenhouse, Margaret Starostik, and Rebecca Tay) and Tatjana Trcek for helpful suggestions. We also thank Himani Galagali and Angela Anderson for comments on the manuscript. This work was supported by a grant from the NIH (R01GM129301) and a grant from the National Institute of Neurological Disorders and Stroke, National Alzheimer{\textquoteright}s Project Act (NAPA) 1-RF1-NS113636-01. Publisher Copyright: {\textcopyright} 2020, eLife Sciences Publications Ltd. All rights reserved.",
year = "2020",
month = apr,
doi = "10.7554/eLife.48480",
language = "English (US)",
volume = "9",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}