Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments

Yan He, Franto Francis, Kenneth A. Myers, Wenqian Yu, Mark M. Black, Peter W. Baas

Research output: Contribution to journalArticlepeer-review

183 Scopus citations


Recent studies have shown that the transport of microtubules (MTs) and neurofilaments (NFs) within the axon is rapid, infrequent, asynchronous, and bidirectional. Here, we used RNA interference to investigate the role of cytoplasmic dynein in powering these transport events. To reveal transport of MTs and NFs, we expressed EGFP-tagged tubulin or NF proteins in cultured rat sympathetic neurons and performed live-cell imaging of the fluorescent cytoskeletal elements in photobleached regions of the axon. The occurrence of anterograde MT and retrograde NF movements was significantly diminished in neurons that had been depleted of dynein heavy chain, whereas the occurrence of retrograde MT and anterograde NF movements was unaffected. These results support a cargo model for NF transport and a sliding filament model for MT transport.

Original languageEnglish (US)
Pages (from-to)697-703
Number of pages7
JournalJournal of Cell Biology
Issue number5
StatePublished - Feb 28 2005

ASJC Scopus subject areas

  • Cell Biology


Dive into the research topics of 'Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments'. Together they form a unique fingerprint.

Cite this