Role of N-myc in the developing mouse kidney

Carlton M. Bates, Sadeq Kharzai, Trent Erwin, Janet Rossant, Luis F. Parada

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

N-myc is a transcription factor expressed in the developing metanephric kidney and other organs. In mice, complete disruption of the N-myc gene results in fetal death on the first day of renal organogenesis. In addition to the null N-myc allele, others have generated a hypomorphic N-myc allele. In this study, combinations of these mutant genes were used to demonstrate that reduction in N-myc protein levels correlate with fewer developing glomeruli and collecting ducts in embryonic kidney explants. Histological sections revealed that the mutant kidneys were hypoplastic with normal developing structures. The data indicate that the hypoplasia is due to a reduction in proliferation rather than an increase in apoptosis. Thus, N-myc loss causes a decrease in numbers of ureteric bud tips and developing glomeruli in explants and hypoplastic kidneys in vivo, in a dose-dependent manner. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)317-325
Number of pages9
JournalDevelopmental Biology
Volume222
Issue number2
DOIs
StatePublished - Jun 15 2000

Keywords

  • Apoptosis
  • Development
  • Kidney
  • N-myc
  • Proliferation

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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    Bates, C. M., Kharzai, S., Erwin, T., Rossant, J., & Parada, L. F. (2000). Role of N-myc in the developing mouse kidney. Developmental Biology, 222(2), 317-325. https://doi.org/10.1006/dbio.2000.9716