Role of neurotrophin-4/5 in neural cell death during retinal development and ischemic retinal injury in vivo

Chikako Harada, Takayuki Harada, Hun Meng A Quah, Kazuhiko Namekata, Kazuhiko Yoshida, Shigeaki Ohno, Kohichi Tanaka, Luis F. Parada

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

PURPOSE. Neurotrophin (NT)-4/5 and brain-derived neurotrophic factor (BDNF) mediate cell survival through TrkB, a high-affinity tyrosine kinase receptor, and may prevent neural cell death in various pathologic conditions. This study was conducted to investigate the function of NT-4/5 in neural cell death during retinal development and ischemic retinal injury. METHODS. Retinal development in wild-type, NT-4/5 knockout (KO), and NT-4/5:BDNF double-KO mice was histologically examined from postnatal day 0 (P0) to P90. Ischemic retinal injury was performed at P42, and NT-4/5 mRNA expression level and the extent of retinal cell death was quantitatively examined. RESULTS. Real-time PCR analysis revealed increased NT-4/5 mRNA expression in the ischemic retina. In the NT-4/5 KO mouse, retinal development and structure were normal, but the strain was susceptible to ischemic injury on P42. In contrast, NT-4/5:BDNF double-KO mice showed delayed retinal development and died before P42. CONCLUSIONS. These results suggest that NT-4/5, in combination with other trophic factors, is involved in the postnatal survival of retinal neurons during both development and degeneration.

Original languageEnglish (US)
Pages (from-to)669-673
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume46
Issue number2
DOIs
StatePublished - Feb 2005

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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    Harada, C., Harada, T., Quah, H. M. A., Namekata, K., Yoshida, K., Ohno, S., Tanaka, K., & Parada, L. F. (2005). Role of neurotrophin-4/5 in neural cell death during retinal development and ischemic retinal injury in vivo. Investigative Ophthalmology and Visual Science, 46(2), 669-673. https://doi.org/10.1167/iovs.04-0826