ROS-containing endosomal compartments: Implications for signaling

A. Paige Davis Volk, Jessica G. Moreland

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

The endogenous generation of reactive oxygen species (ROS), previously perceived as a detrimental by-product of cellular processes, is now recognized as a critical component of intracellular signaling. Exploration of these biological signaling functions requires understanding the complex redox biochemistry and recognizing the compartment-specific elements of ROS generation. The endosomal compartment is increasingly recognized as a source for NADPH oxidase (NOX)-generated signaling ROS. Despite this growing understanding, there are significant limitations to the available detection and measurement systems for endogenous ROS. This chapter provides information about specific methodologies and redox-sensitive probes to guide the investigator and define the critical limitations for many of the available approaches. Although measurement continues to be challenging, the rapid growth and development of new detection systems suggests that our capacity to assign specific signaling roles to endosomal ROS will expand markedly in the next several years.

Original languageEnglish (US)
Title of host publicationEndosome Signaling Part B
PublisherAcademic Press Inc.
Pages201-224
Number of pages24
ISBN (Print)9780123979254
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume535
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

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Keywords

  • Endosome
  • Intracellular signaling
  • NADPH oxidase
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Paige Davis Volk, A., & Moreland, J. G. (2014). ROS-containing endosomal compartments: Implications for signaling. In Endosome Signaling Part B (pp. 201-224). (Methods in Enzymology; Vol. 535). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-397925-4.00013-4