Scanning electron microscopy of Barrett's epithelium and its correlation with light microscopy and mucin stains

Felice Zwas; Helen M. Shields; Wilhelm G. Doos; Donald A. Antonioli; Harvey Goldman; Bernard J. Ransil; Stuart J. Spechler

doi:10.1016/0016-5085(86)90264-7

Scanning electron microscopy of Barrett's epithelium and its correlation with light microscopy and mucin stains

Felice Zwas, Helen M. Shields, Wilhelm G. Doos, Donald A. Antonioli, Harvey Goldman, Bernard J. Ransil, Stuart J. Spechler

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Abstract

The surface epithelial cells of Barrett's esophagus were characterized using quantitative scanning electron microscopy and light microscopy with mucin histochemical stains. Fifty-one biopsy specimens of Barrett's esophagus from 15 patients and 31 control specimens of the stomach and intestines from 9 patients were examined. Three distinct surface cell types, in addition to the goblet cell, were recognized in Barrett's epithelium: the gastric-like cell in 31% of specimens, which was similar to the normal gastric surface cell by quantitative scanning electron microscopy; the intestinal-like cell in 41%, which was most similar to the normal small intestinal surface cell; and the variant cell in 80%, which had a range of surface features. By light microscopy, all specimens with variant and intestinal-like cells were classified as specialized columnar epithelium. The surface mucous cells in Barrett's epithelium displayed a variety of mucin staining patterns with acid nonsulfated (small intestinal-like) mucin present in 90% of specimens and acid sulfated (coloniclike) mucin in 43% of specimens. Quantitative scanning electron microscopy and mucin histochemical stains reveal a striking cellular heterogeneity not apparent by routine light microscopy.

Original language	English (US)
Pages (from-to)	1932-1941
Number of pages	10
Journal	Gastroenterology
Volume	90
Issue number	6
DOIs	https://doi.org/10.1016/0016-5085(86)90264-7
State	Published - Jun 1986

ASJC Scopus subject areas

Hepatology
Gastroenterology

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10.1016/0016-5085(86)90264-7

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@article{2b201657b4d44c86a19ad968a3a34d72,

title = "Scanning electron microscopy of Barrett's epithelium and its correlation with light microscopy and mucin stains",

abstract = "The surface epithelial cells of Barrett's esophagus were characterized using quantitative scanning electron microscopy and light microscopy with mucin histochemical stains. Fifty-one biopsy specimens of Barrett's esophagus from 15 patients and 31 control specimens of the stomach and intestines from 9 patients were examined. Three distinct surface cell types, in addition to the goblet cell, were recognized in Barrett's epithelium: the gastric-like cell in 31% of specimens, which was similar to the normal gastric surface cell by quantitative scanning electron microscopy; the intestinal-like cell in 41%, which was most similar to the normal small intestinal surface cell; and the variant cell in 80%, which had a range of surface features. By light microscopy, all specimens with variant and intestinal-like cells were classified as specialized columnar epithelium. The surface mucous cells in Barrett's epithelium displayed a variety of mucin staining patterns with acid nonsulfated (small intestinal-like) mucin present in 90% of specimens and acid sulfated (coloniclike) mucin in 43% of specimens. Quantitative scanning electron microscopy and mucin histochemical stains reveal a striking cellular heterogeneity not apparent by routine light microscopy.",

author = "Felice Zwas and Shields, {Helen M.} and Doos, {Wilhelm G.} and Antonioli, {Donald A.} and Harvey Goldman and Ransil, {Bernard J.} and Spechler, {Stuart J.}",

note = "Funding Information: Received July 8, 1985. Accepted December 3, 1985. Address requests for reprints to: Felice Zwas, M.D., Division of Gastroenterology, Long Island College Hospital, 340 Henry Street, Brooklyn, New York 11201. This work was supported in part by a Biomedical Research Support Award from the Beth Israel Hospital, Boston, Massachusetts. This work was presented in part at the annual meeting of the American Gastroenterological Association, May 13, 1985. The authors thank Cynthia J. Best for expert technical assistance, Dr. Paul Davies for his advice on morphometric analyses, Dr. Raj Goyal for his advice and support, and Jackie Solberg for help in the preparation of this manuscript. 0 1986 by the American Gastroenterological Association 0016-5085/86/$3.50",

year = "1986",

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T1 - Scanning electron microscopy of Barrett's epithelium and its correlation with light microscopy and mucin stains

AU - Zwas, Felice

AU - Shields, Helen M.

AU - Doos, Wilhelm G.

AU - Antonioli, Donald A.

AU - Goldman, Harvey

AU - Ransil, Bernard J.

AU - Spechler, Stuart J.

N1 - Funding Information: Received July 8, 1985. Accepted December 3, 1985. Address requests for reprints to: Felice Zwas, M.D., Division of Gastroenterology, Long Island College Hospital, 340 Henry Street, Brooklyn, New York 11201. This work was supported in part by a Biomedical Research Support Award from the Beth Israel Hospital, Boston, Massachusetts. This work was presented in part at the annual meeting of the American Gastroenterological Association, May 13, 1985. The authors thank Cynthia J. Best for expert technical assistance, Dr. Paul Davies for his advice on morphometric analyses, Dr. Raj Goyal for his advice and support, and Jackie Solberg for help in the preparation of this manuscript. 0 1986 by the American Gastroenterological Association 0016-5085/86/$3.50

PY - 1986/6

Y1 - 1986/6

N2 - The surface epithelial cells of Barrett's esophagus were characterized using quantitative scanning electron microscopy and light microscopy with mucin histochemical stains. Fifty-one biopsy specimens of Barrett's esophagus from 15 patients and 31 control specimens of the stomach and intestines from 9 patients were examined. Three distinct surface cell types, in addition to the goblet cell, were recognized in Barrett's epithelium: the gastric-like cell in 31% of specimens, which was similar to the normal gastric surface cell by quantitative scanning electron microscopy; the intestinal-like cell in 41%, which was most similar to the normal small intestinal surface cell; and the variant cell in 80%, which had a range of surface features. By light microscopy, all specimens with variant and intestinal-like cells were classified as specialized columnar epithelium. The surface mucous cells in Barrett's epithelium displayed a variety of mucin staining patterns with acid nonsulfated (small intestinal-like) mucin present in 90% of specimens and acid sulfated (coloniclike) mucin in 43% of specimens. Quantitative scanning electron microscopy and mucin histochemical stains reveal a striking cellular heterogeneity not apparent by routine light microscopy.

AB - The surface epithelial cells of Barrett's esophagus were characterized using quantitative scanning electron microscopy and light microscopy with mucin histochemical stains. Fifty-one biopsy specimens of Barrett's esophagus from 15 patients and 31 control specimens of the stomach and intestines from 9 patients were examined. Three distinct surface cell types, in addition to the goblet cell, were recognized in Barrett's epithelium: the gastric-like cell in 31% of specimens, which was similar to the normal gastric surface cell by quantitative scanning electron microscopy; the intestinal-like cell in 41%, which was most similar to the normal small intestinal surface cell; and the variant cell in 80%, which had a range of surface features. By light microscopy, all specimens with variant and intestinal-like cells were classified as specialized columnar epithelium. The surface mucous cells in Barrett's epithelium displayed a variety of mucin staining patterns with acid nonsulfated (small intestinal-like) mucin present in 90% of specimens and acid sulfated (coloniclike) mucin in 43% of specimens. Quantitative scanning electron microscopy and mucin histochemical stains reveal a striking cellular heterogeneity not apparent by routine light microscopy.

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SN - 0016-5085

VL - 90

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EP - 1941

JO - Gastroenterology

JF - Gastroenterology

IS - 6

ER -

Scanning electron microscopy of Barrett's epithelium and its correlation with light microscopy and mucin stains

Abstract

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