Secretome of the biocontrol agent Metarhizium anisopliae induced by the cuticle of the cotton pest Dysdercus peruvianus reveals new insights into infection

Walter O. Beys-Da-Silva, Lucélia Santi, Markus Berger, Diego Calzolari, Dario O. Passos, Jorge A. Guimarães, James J. Moresco, John R. Yates

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Metarhizium anisopliae is an entomopathogenic fungus that has evolved specialized strategies to infect insect hosts. Here we analyzed secreted proteins related to Dysdercus peruvianus infection. Using shotgun proteomics, abundance changes in 71 proteins were identified after exposure to host cuticle. Among these proteins were classical fungal effectors secreted by pathogens to degrade physical barriers and alter host physiology. These include lipolytic enzymes, Pr1A, B, C, I, and J proteases, ROS-related proteins, oxidorreductases, and signaling proteins. Protein interaction networks were generated postulating interesting candidates for further studies, including Pr1C, based on possible functional interactions. On the basis of these results, we propose that M. anisopliae is degrading host components and actively secreting proteins to manage the physiology of the host. Interestingly, the secretion of these factors occurs in the absence of a host response. The findings presented here are an important step in understanding the host-pathogen interaction and developing more efficient biocontrol of D. peruvianus by M. anisopliae.

Original languageEnglish (US)
Pages (from-to)2282-2296
Number of pages15
JournalJournal of Proteome Research
Volume13
Issue number5
DOIs
StatePublished - May 2 2014
Externally publishedYes

Fingerprint

Metarhizium
Biocontrol
Cotton
Infection
Proteins
Physiology
Pathogens
Protein Interaction Maps
Host-Pathogen Interactions
Architectural Accessibility
Firearms
Proteomics
Insects
Fungi
Peptide Hydrolases
Enzymes

Keywords

  • biocontrol
  • Dysdercus peruvianus
  • host infection
  • Metarhizium anisopliae
  • shotgun proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Secretome of the biocontrol agent Metarhizium anisopliae induced by the cuticle of the cotton pest Dysdercus peruvianus reveals new insights into infection. / Beys-Da-Silva, Walter O.; Santi, Lucélia; Berger, Markus; Calzolari, Diego; Passos, Dario O.; Guimarães, Jorge A.; Moresco, James J.; Yates, John R.

In: Journal of Proteome Research, Vol. 13, No. 5, 02.05.2014, p. 2282-2296.

Research output: Contribution to journalArticle

Beys-Da-Silva, Walter O. ; Santi, Lucélia ; Berger, Markus ; Calzolari, Diego ; Passos, Dario O. ; Guimarães, Jorge A. ; Moresco, James J. ; Yates, John R. / Secretome of the biocontrol agent Metarhizium anisopliae induced by the cuticle of the cotton pest Dysdercus peruvianus reveals new insights into infection. In: Journal of Proteome Research. 2014 ; Vol. 13, No. 5. pp. 2282-2296.
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