Seeing is believing: Dynamic changes in renal epithelial autophagy during injury and repair

Fangming Lin; Zhao Wang; Joseph A Hill

doi:10.4161/auto.27749

Seeing is believing: Dynamic changes in renal epithelial autophagy during injury and repair

Fangming Lin, Zhao Wang, Joseph A Hill

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Ischemic injury to the kidneys is a prevalent clinical problem, contributing importantly to chronic kidney disease. Yet, underlying molecular mechanisms are elusive. To address the possible role of autophagy, we engineered a novel strain of mice harboring a ubiquitously expressed CAG-RFP-EGFP-LC3 transgene. Using this tool, we examined the post-ischemic kidney and detailed the dynamics of renal tubular epithelial autophagy. In addition, we defined the role of MTOR in the resolution of autophagy during epithelial survival and kidney repair.

Original language	English (US)
Pages (from-to)	691-693
Number of pages	3
Journal	Autophagy
Volume	10
Issue number	4
DOIs	https://doi.org/10.4161/auto.27749
State	Published - Apr 2014

Keywords

Dymanics
Ischemic injury
Renal epithelial cells
Renal repair
Tandem fluorescence

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.4161/auto.27749

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title = "Seeing is believing: Dynamic changes in renal epithelial autophagy during injury and repair",

abstract = "Ischemic injury to the kidneys is a prevalent clinical problem, contributing importantly to chronic kidney disease. Yet, underlying molecular mechanisms are elusive. To address the possible role of autophagy, we engineered a novel strain of mice harboring a ubiquitously expressed CAG-RFP-EGFP-LC3 transgene. Using this tool, we examined the post-ischemic kidney and detailed the dynamics of renal tubular epithelial autophagy. In addition, we defined the role of MTOR in the resolution of autophagy during epithelial survival and kidney repair.",

keywords = "Dymanics, Ischemic injury, Renal epithelial cells, Renal repair, Tandem fluorescence",

author = "Fangming Lin and Zhao Wang and Hill, {Joseph A}",

note = "Funding Information: This work was supported by the NIH grant R01DK083411 and a March of Dimes research grant to FL; and grants from the NIH (HL-080144, HL-0980842, and HL-100401), CPRIT (RP110486P3), the AHA-DeHaan Foundation (0970518N), and the Fondation Leducq (11CVD04) to JAH. FL is a Samberg Scholar in Children{\textquoteright}s Health at Morgan Stanley Children{\textquoteright}s Hospital of the New York Presbyterian Hospital.",

year = "2014",

month = apr,

doi = "10.4161/auto.27749",

language = "English (US)",

volume = "10",

pages = "691--693",

journal = "Autophagy",

issn = "1554-8627",

publisher = "Landes Bioscience",

number = "4",

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TY - JOUR

T1 - Seeing is believing

T2 - Dynamic changes in renal epithelial autophagy during injury and repair

AU - Lin, Fangming

AU - Wang, Zhao

AU - Hill, Joseph A

N1 - Funding Information: This work was supported by the NIH grant R01DK083411 and a March of Dimes research grant to FL; and grants from the NIH (HL-080144, HL-0980842, and HL-100401), CPRIT (RP110486P3), the AHA-DeHaan Foundation (0970518N), and the Fondation Leducq (11CVD04) to JAH. FL is a Samberg Scholar in Children’s Health at Morgan Stanley Children’s Hospital of the New York Presbyterian Hospital.

PY - 2014/4

Y1 - 2014/4

N2 - Ischemic injury to the kidneys is a prevalent clinical problem, contributing importantly to chronic kidney disease. Yet, underlying molecular mechanisms are elusive. To address the possible role of autophagy, we engineered a novel strain of mice harboring a ubiquitously expressed CAG-RFP-EGFP-LC3 transgene. Using this tool, we examined the post-ischemic kidney and detailed the dynamics of renal tubular epithelial autophagy. In addition, we defined the role of MTOR in the resolution of autophagy during epithelial survival and kidney repair.

AB - Ischemic injury to the kidneys is a prevalent clinical problem, contributing importantly to chronic kidney disease. Yet, underlying molecular mechanisms are elusive. To address the possible role of autophagy, we engineered a novel strain of mice harboring a ubiquitously expressed CAG-RFP-EGFP-LC3 transgene. Using this tool, we examined the post-ischemic kidney and detailed the dynamics of renal tubular epithelial autophagy. In addition, we defined the role of MTOR in the resolution of autophagy during epithelial survival and kidney repair.

KW - Dymanics

KW - Ischemic injury

KW - Renal epithelial cells

KW - Renal repair

KW - Tandem fluorescence

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JO - Autophagy

JF - Autophagy

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ER -

Seeing is believing: Dynamic changes in renal epithelial autophagy during injury and repair

Abstract

Keywords

ASJC Scopus subject areas

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