Selective potentiation of a novel calcium channel in rat hippocampal neurones

E. T. Kavalali, M. R. Plummer

Research output: Contribution to journalArticle

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Abstract

1. Calcium channel activity in cultured embryonic hippocampal neurones was studied with the cell-attached configuration of the patch clamp technique. Single-channel recordings revealed the presence of a novel kind of calcium channel activity characterized by marked bursts of re-openings following voltage pulses to +20 mV from a holding potential of -40 mV. 2. The re-openings were greatly prolonged by the dihydropyridine (DHP) agonist (+)-(S)-202-791, thus ruling out the possibility that they arose from T-, N- or P-type channels. Furthermore, the novel gating pattern could be readily distinguished from that of the L-type channel which showed only conventional tail currents. 3. Since the novel gating pattern was stable over many minutes, we provisionally referred to it as a novel kind of calcium channel that showed voltage-dependent potentiation (L(p) channel) to distinguish it from the 'standard' L-type channel (L(s) channel). 4. L(p) channels could also be distinguished from L(s) channels on the basis of slope conductance (24.3 vs. 26.9 pS for L(p) and L(s), respectively) and mean DHP-induced long open time (2.7 vs. 11 ms at +20 mV for L(p) and L(s), respectively). 5. Voltage-dependent potentiation of L(p) channel activity was studied using a dual-pulse protocol. When preceded by conditioning prepulses, L(p) responses to test pulses were greatly increased. L(s)- and N-type calcium channels showed no such enhancement of their activity. 6. Long-duration recordings revealed no clear evidence for transitions from L(s) to L(p) gating (or vice versa), suggesting that L(s) and L(p) activities arose from different kinds of calcium channels or that L(p) gating is an unusually long-lived mode of L(s) channel gating.

Original languageEnglish (US)
Pages (from-to)475-484
Number of pages10
JournalJournal of Physiology
Volume480
Issue number3
StatePublished - 1994

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Calcium Channels
Neurons
N-Type Calcium Channels
Patch-Clamp Techniques
Tail
1,4-dihydropyridine

ASJC Scopus subject areas

  • Physiology

Cite this

Selective potentiation of a novel calcium channel in rat hippocampal neurones. / Kavalali, E. T.; Plummer, M. R.

In: Journal of Physiology, Vol. 480, No. 3, 1994, p. 475-484.

Research output: Contribution to journalArticle

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N2 - 1. Calcium channel activity in cultured embryonic hippocampal neurones was studied with the cell-attached configuration of the patch clamp technique. Single-channel recordings revealed the presence of a novel kind of calcium channel activity characterized by marked bursts of re-openings following voltage pulses to +20 mV from a holding potential of -40 mV. 2. The re-openings were greatly prolonged by the dihydropyridine (DHP) agonist (+)-(S)-202-791, thus ruling out the possibility that they arose from T-, N- or P-type channels. Furthermore, the novel gating pattern could be readily distinguished from that of the L-type channel which showed only conventional tail currents. 3. Since the novel gating pattern was stable over many minutes, we provisionally referred to it as a novel kind of calcium channel that showed voltage-dependent potentiation (L(p) channel) to distinguish it from the 'standard' L-type channel (L(s) channel). 4. L(p) channels could also be distinguished from L(s) channels on the basis of slope conductance (24.3 vs. 26.9 pS for L(p) and L(s), respectively) and mean DHP-induced long open time (2.7 vs. 11 ms at +20 mV for L(p) and L(s), respectively). 5. Voltage-dependent potentiation of L(p) channel activity was studied using a dual-pulse protocol. When preceded by conditioning prepulses, L(p) responses to test pulses were greatly increased. L(s)- and N-type calcium channels showed no such enhancement of their activity. 6. Long-duration recordings revealed no clear evidence for transitions from L(s) to L(p) gating (or vice versa), suggesting that L(s) and L(p) activities arose from different kinds of calcium channels or that L(p) gating is an unusually long-lived mode of L(s) channel gating.

AB - 1. Calcium channel activity in cultured embryonic hippocampal neurones was studied with the cell-attached configuration of the patch clamp technique. Single-channel recordings revealed the presence of a novel kind of calcium channel activity characterized by marked bursts of re-openings following voltage pulses to +20 mV from a holding potential of -40 mV. 2. The re-openings were greatly prolonged by the dihydropyridine (DHP) agonist (+)-(S)-202-791, thus ruling out the possibility that they arose from T-, N- or P-type channels. Furthermore, the novel gating pattern could be readily distinguished from that of the L-type channel which showed only conventional tail currents. 3. Since the novel gating pattern was stable over many minutes, we provisionally referred to it as a novel kind of calcium channel that showed voltage-dependent potentiation (L(p) channel) to distinguish it from the 'standard' L-type channel (L(s) channel). 4. L(p) channels could also be distinguished from L(s) channels on the basis of slope conductance (24.3 vs. 26.9 pS for L(p) and L(s), respectively) and mean DHP-induced long open time (2.7 vs. 11 ms at +20 mV for L(p) and L(s), respectively). 5. Voltage-dependent potentiation of L(p) channel activity was studied using a dual-pulse protocol. When preceded by conditioning prepulses, L(p) responses to test pulses were greatly increased. L(s)- and N-type calcium channels showed no such enhancement of their activity. 6. Long-duration recordings revealed no clear evidence for transitions from L(s) to L(p) gating (or vice versa), suggesting that L(s) and L(p) activities arose from different kinds of calcium channels or that L(p) gating is an unusually long-lived mode of L(s) channel gating.

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