Separable enhancer sequences regulate the expression of the neural bHLH transcription factor neurogenin 1

Yuji Nakada, Preeti Parab, Andrew Simmons, Adil Omer-Abdalla, Jane E. Johnson

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Ngn1 is a basic helix-loop-helix (bHLH) transcription factor expressed in specific regions within the developing brain and spinal cord, sensory ganglia, and olfactory epithelium. We have identified sequences both 5′ and 3′ of the mouse ngn1 gene that function in regulating ngn1 expression, and each of these sequences contains distinct regulatory cassettes for different subregions of the expression domain. Enhancers for expression in ngn1 domains of the midbrain, hindbrain, trigeminal ganglia, and ventral-neural tube appear redundant and are spread both 5′ and 3′ of the ngn1 coding sequence. In contrast, a single discrete dorsal-neural tube enhancer was located in the 5′ sequence that is conserved among mouse, human, chick, and zebrafish ngn1 genes. Functionally, this enhancer is both necessary and sufficient for driving expression of a heterologous reporter in transgenic mice specifically to the dorsal domain of ngn1 expression in the spinal neural tube. Thus, sequences are identified that can be used to direct temporally and spatially restricted expression of heterologous genes to the developing neural tube.

Original languageEnglish (US)
Pages (from-to)479-487
Number of pages9
JournalDevelopmental Biology
Volume271
Issue number2
DOIs
StatePublished - Jul 15 2004

Keywords

  • Basic helix-loop-helix transcription factor
  • Dorsal-neural tube
  • Neurogenin 1
  • Spinal cord development
  • Transcriptional regulation
  • Transgenic mice
  • Ventral-neural tube

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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