Sephadex G-10 adsorption chromatography of purines and related compounds

Lawrence Sweetman, William L. Nyhan

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

The adsorption chromatography of purines and related heterocyclic compounds on Sephadex G-10 was investigated under different conditions of pH, ionic strength and flow rate and found to give excellent separations. The elution volumes were highly reproducible and recovery quantitative. A relation between a log function of the elution volumes and substituents on the purine ring was shown and the additivity of the resulting Δ log V6° values used to predict elution volumes. Some analytical and preparative applications have been undertaken.

Original languageEnglish (US)
Pages (from-to)662-675
Number of pages14
JournalJournal of Chromatography A
Volume32
Issue numberC
DOIs
StatePublished - 1968
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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