Sequence diversity in genes of lipid metabolism

Christine Kim Garcia, Gabriele Mues, Yuanlan Liao, Tommy Hyatt, Nila Patil, Jonathan C. Cohen, Helen H. Hobbs

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Elevated plasma lipoprotein levels play a crucial role in the development of coronary artery disease. Genetic factors strongly influence the levels of plasma lipoproteins, but the genes and sequence variations contributing to the most common forms of dyslipidemias are not known. We used GeneChip probe arrays to resequence the coding regions of 10 key genes of lipid metabolism. The sequences of these genes were analyzed in 80 dyslipidemic individuals. Fourteen nonsynonymous and twenty-two synonymous single nucleotide changes were identified that could be confirmed by conventional sequencing. Seven of the fourteen nonsynonymous sequence variants were polymorphisms with allele frequency >1% in the general population. The remaining seven were not found in normolipidemic controls (25 Caucasians and 25 African-Americans). The relationship between nonsynonymous sequence variations and various dyslipidemias was explored in association and family studies. No evidence was found for coding sequence variations in any of the 10 genes contributing to dyslipidemia. Only a single sequence variation, a missense mutation in the low density lipoprotein receptor gene, co-segregated with hyperlipidemia in the proband's family. This study illustrates some of the difficulties associated with identifying sequence variations contributing to complex traits.

Original languageEnglish (US)
Pages (from-to)1043-1052
Number of pages10
JournalGenome Research
Volume11
Issue number6
DOIs
StatePublished - 2001

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Lipid Metabolism
Dyslipidemias
Genes
Lipoproteins
LDL Receptors
Missense Mutation
Hyperlipidemias
Gene Frequency
African Americans
Coronary Artery Disease
Nucleotides
Population

ASJC Scopus subject areas

  • Genetics

Cite this

Garcia, C. K., Mues, G., Liao, Y., Hyatt, T., Patil, N., Cohen, J. C., & Hobbs, H. H. (2001). Sequence diversity in genes of lipid metabolism. Genome Research, 11(6), 1043-1052. https://doi.org/10.1101/gr.172301

Sequence diversity in genes of lipid metabolism. / Garcia, Christine Kim; Mues, Gabriele; Liao, Yuanlan; Hyatt, Tommy; Patil, Nila; Cohen, Jonathan C.; Hobbs, Helen H.

In: Genome Research, Vol. 11, No. 6, 2001, p. 1043-1052.

Research output: Contribution to journalArticle

Garcia, CK, Mues, G, Liao, Y, Hyatt, T, Patil, N, Cohen, JC & Hobbs, HH 2001, 'Sequence diversity in genes of lipid metabolism', Genome Research, vol. 11, no. 6, pp. 1043-1052. https://doi.org/10.1101/gr.172301
Garcia CK, Mues G, Liao Y, Hyatt T, Patil N, Cohen JC et al. Sequence diversity in genes of lipid metabolism. Genome Research. 2001;11(6):1043-1052. https://doi.org/10.1101/gr.172301
Garcia, Christine Kim ; Mues, Gabriele ; Liao, Yuanlan ; Hyatt, Tommy ; Patil, Nila ; Cohen, Jonathan C. ; Hobbs, Helen H. / Sequence diversity in genes of lipid metabolism. In: Genome Research. 2001 ; Vol. 11, No. 6. pp. 1043-1052.
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