Sex-chromosome dosage effects on gene expression in humans

Armin Raznahan, Neelroop N. Parikshak, Vijay Chandran, Jonathan D. Blumenthal, Liv S. Clasen, Aaron F. Alexander-Bloch, Andrew R. Zinn, Danny Wangsa, Jasen Wise, Declan G.M. Murphy, Patrick F. Bolton, Thomas Ried, Judith Ross, Jay N. Giedd, Daniel H. Geschwind

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A fundamental question in the biology of sex differences has eluded direct study in humans: How does sex-chromosome dosage (SCD) shape genome function? To address this, we developed a systematic map of SCD effects on gene function by analyzing genome-wide expression data in humans with diverse sex-chromosome aneuploidies (XO, XXX, XXY, XYY, and XXYY). For sex chromosomes, we demonstrate a pattern of obligate dosage sensitivity among evolutionarily preserved X-Y homologs and update prevailing theoretical models for SCD compensation by detecting X-linked genes that increase expression with decreasing X- and/or Y-chromosome dosage. We further show that SCD-sensitive sex-chromosome genes regulate specific coexpression networks of SCD-sensitive autosomal genes with critical cellular functions and a demonstrable potential to mediate previously documented SCD effects on disease. These gene coexpression results converge with analysis of transcription factor binding site enrichment and measures of gene expression in murine knockout models to spotlight the dosage-sensitive X-linked transcription factor ZFX as a key mediator of SCD effects on wider genome expression. Our findings characterize the effects of SCD broadly across the genome, with potential implications for human phenotypic variation.

Original languageEnglish (US)
Pages (from-to)7398-7403
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number28
DOIs
StatePublished - Jul 10 2018

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Sex Chromosomes
Gene Expression
Genome
Genes
Transcription Factors
X-Linked Genes
Y Chromosome
Aneuploidy
Sex Characteristics
Theoretical Models
Binding Sites

Keywords

  • Klinefelter syndrome
  • Sex chromosomes
  • Sex differences
  • Turner syndrome
  • X-inactivation

ASJC Scopus subject areas

  • General

Cite this

Raznahan, A., Parikshak, N. N., Chandran, V., Blumenthal, J. D., Clasen, L. S., Alexander-Bloch, A. F., ... Geschwind, D. H. (2018). Sex-chromosome dosage effects on gene expression in humans. Proceedings of the National Academy of Sciences of the United States of America, 115(28), 7398-7403. https://doi.org/10.1073/pnas.1802889115

Sex-chromosome dosage effects on gene expression in humans. / Raznahan, Armin; Parikshak, Neelroop N.; Chandran, Vijay; Blumenthal, Jonathan D.; Clasen, Liv S.; Alexander-Bloch, Aaron F.; Zinn, Andrew R.; Wangsa, Danny; Wise, Jasen; Murphy, Declan G.M.; Bolton, Patrick F.; Ried, Thomas; Ross, Judith; Giedd, Jay N.; Geschwind, Daniel H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 28, 10.07.2018, p. 7398-7403.

Research output: Contribution to journalArticle

Raznahan, A, Parikshak, NN, Chandran, V, Blumenthal, JD, Clasen, LS, Alexander-Bloch, AF, Zinn, AR, Wangsa, D, Wise, J, Murphy, DGM, Bolton, PF, Ried, T, Ross, J, Giedd, JN & Geschwind, DH 2018, 'Sex-chromosome dosage effects on gene expression in humans', Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 28, pp. 7398-7403. https://doi.org/10.1073/pnas.1802889115
Raznahan A, Parikshak NN, Chandran V, Blumenthal JD, Clasen LS, Alexander-Bloch AF et al. Sex-chromosome dosage effects on gene expression in humans. Proceedings of the National Academy of Sciences of the United States of America. 2018 Jul 10;115(28):7398-7403. https://doi.org/10.1073/pnas.1802889115
Raznahan, Armin ; Parikshak, Neelroop N. ; Chandran, Vijay ; Blumenthal, Jonathan D. ; Clasen, Liv S. ; Alexander-Bloch, Aaron F. ; Zinn, Andrew R. ; Wangsa, Danny ; Wise, Jasen ; Murphy, Declan G.M. ; Bolton, Patrick F. ; Ried, Thomas ; Ross, Judith ; Giedd, Jay N. ; Geschwind, Daniel H. / Sex-chromosome dosage effects on gene expression in humans. In: Proceedings of the National Academy of Sciences of the United States of America. 2018 ; Vol. 115, No. 28. pp. 7398-7403.
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