Shift in Kiss1 cell activity requires estrogen receptor

Renata Frazão, Roberta M. Cravo, Jose Donato, Dhirender V. Ratra, Deborah J. Clegg, Joel K. Elmquist, Jeffrey M. Zigman, Kevin W. Williams, Carol F. Elias

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Reproductive function requires timely secretion of gonadotropin-releasing hormone, which is controlled by a complex excitatory/ inhibitory network influenced by sex steroids. Kiss1 neurons are fundamental players in this network, but it is currently unclear whether different conditions of circulating sex steroids directly alter Kiss1 neuronal activity. Here, we show that Kiss1 neurons in the anteroventral periventricular and anterior periventricular nuclei (AVPV/PeN) of males and females exhibit a bimodal resting membrane potential (RMP) influenced by KATP channels, suggesting the presence of two neuronal populations defined as type I (irregular firing patterns) and type II (quiescent). Kiss1 neurons in the arcuate nucleus (Arc) are also composed of firing and quiescent cells, but unlike AVPV/PeN neurons, the range of RMPs did not follow a bimodal distribution. Moreover, Kiss1 neuronal activity in the AVPV/PeN, but not in the Arc, is sexually dimorphic. In females, estradiol shifts the firing pattern of AVPV/PeN Kiss1 neurons and alters cell capacitance and spontaneous IPSCs amplitude of AVPV/PeN and Arc Kiss1 populations in an opposite manner. Notably, mice with selective deletion of estrogen receptor α (ERα) from Kiss1 neurons show cellular activity similar to that observed in ovariectomized females, suggesting that estradiol-induced changes in Kiss1 cellular properties require ERα. We also show that female prepubertal Kiss1 neurons are under higher inhibitory influence and all recorded AVPV/PeN Kiss1 neurons were spontaneously active. Collectively, our findings indicate that changes in cellular activity may underlie Kiss1 action in pubertal initiation and female reproduction.

Original languageEnglish (US)
Pages (from-to)2807-2820
Number of pages14
JournalJournal of Neuroscience
Volume33
Issue number7
DOIs
StatePublished - Feb 13 2013

Fingerprint

Estrogen Receptors
Neurons
Estradiol
Steroids
KATP Channels
Arcuate Nucleus of Hypothalamus
Gonadotropin-Releasing Hormone
Membrane Potentials
Population
Reproduction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Frazão, R., Cravo, R. M., Donato, J., Ratra, D. V., Clegg, D. J., Elmquist, J. K., ... Elias, C. F. (2013). Shift in Kiss1 cell activity requires estrogen receptor. Journal of Neuroscience, 33(7), 2807-2820. https://doi.org/10.1523/JNEUROSCI.1610-12.2013

Shift in Kiss1 cell activity requires estrogen receptor. / Frazão, Renata; Cravo, Roberta M.; Donato, Jose; Ratra, Dhirender V.; Clegg, Deborah J.; Elmquist, Joel K.; Zigman, Jeffrey M.; Williams, Kevin W.; Elias, Carol F.

In: Journal of Neuroscience, Vol. 33, No. 7, 13.02.2013, p. 2807-2820.

Research output: Contribution to journalArticle

Frazão, R, Cravo, RM, Donato, J, Ratra, DV, Clegg, DJ, Elmquist, JK, Zigman, JM, Williams, KW & Elias, CF 2013, 'Shift in Kiss1 cell activity requires estrogen receptor', Journal of Neuroscience, vol. 33, no. 7, pp. 2807-2820. https://doi.org/10.1523/JNEUROSCI.1610-12.2013
Frazão, Renata ; Cravo, Roberta M. ; Donato, Jose ; Ratra, Dhirender V. ; Clegg, Deborah J. ; Elmquist, Joel K. ; Zigman, Jeffrey M. ; Williams, Kevin W. ; Elias, Carol F. / Shift in Kiss1 cell activity requires estrogen receptor. In: Journal of Neuroscience. 2013 ; Vol. 33, No. 7. pp. 2807-2820.
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