Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe

X. Saez-Llorens; L. M. Guzman-Verduzco; S. Shelton; J. D. Nelson; Y. M. Kupersztoch

doi:10.1128/jcm.27.7.1684-1688.1989

Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe

X. Saez-Llorens, L. M. Guzman-Verduzco, S. Shelton, J. D. Nelson, Y. M. Kupersztoch

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A single RNA probe was synthesized and used to detect simultaneously the methanol-soluble heat-stable enterotoxin and heat-labile enterotoxin genes in Escherichia coli strains. The results with the biotinylated or radioactive probe correlated 100% with the biological assay results for both toxins. The RNA probe detected the three known heat-stable enterotoxin A alleles.

Original language	English (US)
Pages (from-to)	1684-1688
Number of pages	5
Journal	Journal of clinical microbiology
Volume	27
Issue number	7
DOIs	https://doi.org/10.1128/jcm.27.7.1684-1688.1989
State	Published - 1989

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/jcm.27.7.1684-1688.1989

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title = "Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe",

abstract = "A single RNA probe was synthesized and used to detect simultaneously the methanol-soluble heat-stable enterotoxin and heat-labile enterotoxin genes in Escherichia coli strains. The results with the biotinylated or radioactive probe correlated 100% with the biological assay results for both toxins. The RNA probe detected the three known heat-stable enterotoxin A alleles.",

author = "X. Saez-Llorens and Guzman-Verduzco, {L. M.} and S. Shelton and Nelson, {J. D.} and Kupersztoch, {Y. M.}",

year = "1989",

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T1 - Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe

AU - Saez-Llorens, X.

AU - Guzman-Verduzco, L. M.

AU - Shelton, S.

AU - Nelson, J. D.

AU - Kupersztoch, Y. M.

PY - 1989

Y1 - 1989

N2 - A single RNA probe was synthesized and used to detect simultaneously the methanol-soluble heat-stable enterotoxin and heat-labile enterotoxin genes in Escherichia coli strains. The results with the biotinylated or radioactive probe correlated 100% with the biological assay results for both toxins. The RNA probe detected the three known heat-stable enterotoxin A alleles.

AB - A single RNA probe was synthesized and used to detect simultaneously the methanol-soluble heat-stable enterotoxin and heat-labile enterotoxin genes in Escherichia coli strains. The results with the biotinylated or radioactive probe correlated 100% with the biological assay results for both toxins. The RNA probe detected the three known heat-stable enterotoxin A alleles.

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DO - 10.1128/jcm.27.7.1684-1688.1989

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AN - SCOPUS:0024353618

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IS - 7

ER -

Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe

Abstract

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