Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe

X. Saez-Llorens, L. M. Guzman-Verduzco, S. Shelton, J. D. Nelson, Y. M. Kupersztoch

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A single RNA probe was synthesized and used to detect simultaneously the methanol-soluble heat-stable enterotoxin and heat-labile enterotoxin genes in Escherichia coli strains. The results with the biotinylated or radioactive probe correlated 100% with the biological assay results for both toxins. The RNA probe detected the three known heat-stable enterotoxin A alleles.

Original languageEnglish (US)
Pages (from-to)1684-1688
Number of pages5
JournalJournal of clinical microbiology
Volume27
Issue number7
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Microbiology (medical)

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    Saez-Llorens, X., Guzman-Verduzco, L. M., Shelton, S., Nelson, J. D., & Kupersztoch, Y. M. (1989). Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe. Journal of clinical microbiology, 27(7), 1684-1688.