Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe

X. Saez-Llorens, L. M. Guzman-Verduzco, S. Shelton, J. D. Nelson, Y. M. Kupersztoch

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A single RNA probe was synthesized and used to detect simultaneously the methanol-soluble heat-stable enterotoxin and heat-labile enterotoxin genes in Escherichia coli strains. The results with the biotinylated or radioactive probe correlated 100% with the biological assay results for both toxins. The RNA probe detected the three known heat-stable enterotoxin A alleles.

Original languageEnglish (US)
Pages (from-to)1684-1688
Number of pages5
JournalJournal of Clinical Microbiology
Volume27
Issue number7
StatePublished - 1989

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RNA Probes
Enterotoxins
Hot Temperature
Escherichia coli
Genes
Biological Assay
Methanol
Alleles

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Saez-Llorens, X., Guzman-Verduzco, L. M., Shelton, S., Nelson, J. D., & Kupersztoch, Y. M. (1989). Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe. Journal of Clinical Microbiology, 27(7), 1684-1688.

Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe. / Saez-Llorens, X.; Guzman-Verduzco, L. M.; Shelton, S.; Nelson, J. D.; Kupersztoch, Y. M.

In: Journal of Clinical Microbiology, Vol. 27, No. 7, 1989, p. 1684-1688.

Research output: Contribution to journalArticle

Saez-Llorens, X, Guzman-Verduzco, LM, Shelton, S, Nelson, JD & Kupersztoch, YM 1989, 'Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe', Journal of Clinical Microbiology, vol. 27, no. 7, pp. 1684-1688.
Saez-Llorens, X. ; Guzman-Verduzco, L. M. ; Shelton, S. ; Nelson, J. D. ; Kupersztoch, Y. M. / Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe. In: Journal of Clinical Microbiology. 1989 ; Vol. 27, No. 7. pp. 1684-1688.
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